The peptide p2Ca is immunodominant in allorecognition of Ld by beta chain variable region V beta 8+ but not V beta 8- strains.
The peptide p2Ca is immunodominant in allorecognition of Ld by beta chain variable region V beta 8+ but not V beta 8- strains.
复制标题
肽p2Ca在β链可变区Vβ8而非Vβ8-菌株对Ld的同种异体识别中具有免疫显性。
DOI：
10.1073/pnas.91.24.11482
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发表时间：
1994
影响因子：
11.1
通讯作者：
Connolly,JM
中科院分区：
文献类型：
--
作者：
Connolly,JM
免疫学家长期以来一直无法解释同种异体移植物强烈排斥反应是由CD 8 + T细胞识别同种异体主要组织相容性复合物（MHC）分子引起的。最近的证据表明，同种异体反应性涉及识别同种异体MHC分子及其相关的肽配体，这表明目前的理论，同种异体反应的强度来自于许多肽的参与。然而，我在这里报告说，一个单一的肽，p2Ca，是免疫显性识别的小鼠MHC I类分子H-2Ld。大多数LD同种异体反应性T细胞克隆对Ld-p2Ca具有特异性，并且这种免疫优势不是由于肽交叉反应性。在对p2Ca耐受的菌株中产生LD同种异体反应性细胞毒性T淋巴细胞不影响肽免疫优势，表明对p2Ca的耐受是MHC限制性的。p2Ca特异性克隆表达β链可变区V β 8 T细胞受体，然而，在V β 8小鼠中产生的LD同种异体反应性细胞毒性T淋巴细胞不受p2Ca识别的支配，这表明T细胞受体库是决定肽免疫显性的因素。
An explanation for the vigorous allograft rejection that results from the recognition by CD8+ T cells of allogeneic major histocompatibility complex (MHC) molecules has long eluded immunologists. Recent evidence has demonstrated that alloreactivity involves recognition of both the allogeneic MHC molecule and its associated peptide ligand, suggesting the current theory that the strength of the allogeneic response results from the participation of numerous peptides. However, I report here that a single peptide, p2Ca, is immunodominant in allorecognition of the murine MHC class I molecule H-2Ld. The majority of Ld-alloreactive T-cell clones are specific for Ld-p2Ca and this immunodominance is not due to peptide cross-reactivity. Generation of Ld-alloreactive cytotoxic T lymphocytes in a strain tolerant to p2Ca did not affect the peptide immunodominance, demonstrating that tolerance to p2Ca is MHC-restricted. The p2Ca-specific clones express beta chain variable region V beta 8 T-cell receptors, however, Ld-alloreactive cytotoxic T lymphocytes generated in V beta 8- mice are not dominated by recognition of p2Ca, suggesting that the T-cell receptor repertoire is a factor in determining peptide immunodominance.