Differential Innervation of Secretory Coils and Ducts in Human Eccrine Sweat Glands
Differential Innervation of Secretory Coils and Ducts in Human Eccrine Sweat Glands
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DOI：
10.4103/0366-6999.238142
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发表时间：
2018-08
影响因子：
6.1
通讯作者：
Zhan Ouyang;Haihong Li;Mingjun Zhang;Sitian Xie;Liu-Hang-Hang Cheng-Liu-Hang-Hang-Cheng-51173527
中科院分区：
文献类型：
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作者：
Zhan Ouyang;Haihong Li;Mingjun Zhang;Sitian Xie;Liu-Hang-Hang Cheng-Liu-Hang-Hang-Cheng-51173527
背景：以往的研究表明，小汗腺既有胆碱能神经也有肾上腺素能神经支配。然而，小汗腺的分泌线圈和导管是否同样受到交感神经纤维的支配还是个未知数。为了更好地了解汗液分泌和重吸收的机制，本研究对人外分泌汗腺的分泌线圈和汗管的分化神经支配进行了研究。方法：从2016年6月至2017年6月，对6例人皮肤标本进行固定、石蜡包埋、切片，采用标准免疫荧光结合酪胺信号扩增(IF-TSA)的方法，对神经纤维标志物蛋白基因产物9.5(PGP 9.5)、酪氨酸羟基酶(TH)、血管活性肠肽(VIP)、汗腺标志物K7、S100P、K14进行共染色。染色切片在显微镜下观察、拍照、分析。结果：IF-TSA能很好地显示汗腺周围PGP 9.5、TH和VIP的荧光信号，而常规IF只能观察到PGP 9.5的荧光信号。IF-TSA法在检测低水平表达的抗原时比标准IF法更敏感。PGP9.5、TH和VIP主要出现在分泌线圈周围，汗管周围稀疏。结论：汗液的分泌主要由自主神经控制，而汗液重吸收受神经活动的影响较小。
Background: Previous studies demonstrate that eccrine sweat glands are innervated by both cholinergic and adrenergic nerves. However, it is still unknown whether the secretory coils and ducts of eccrine sweat glands are equally innervated by the sympathetic nerve fibers. To well understand the mechanisms on sweat secretion and reabsorption, the differential innervation of secretory coils and ducts in human eccrine sweat glands was investigated in the study. Methods: From June 2016 to June 2017, six human skins were fixed, paraffin-embedded, and cut into 5 &mgr;m-thick sections, followed by costaining for nerve fiber markers protein gene product 9.5 (PGP 9.5), tyrosine hydroxylase (TH) and vasoactive intestinal peptide (VIP), and eccrine sweat gland markers K7, S100P, and K14 by combining standard immunofluorescence with tyramide signal amplification (IF-TSA). Stained sections were observed under the microscope, photographed, and analyzed. Results: The fluorescent signals of PGP 9.5, TH, and VIP were easily visualized, by IF-TSA, as circular patterns surrounding eccrine sweat glands, but only PGP 9.5 could be observed by standard IF. The IF-TSA method is more sensitivity than standard IF in detecting antigens expressed at low levels. PGP 9.5, TH, and VIP appeared primarily surrounding the secretory coils and sparsely surrounding the sweat ducts. Conclusion: Sweat secretion is mainly controlled by autonomic nerves whereas sweat reabsorption is less affected by nerve activity.