Effect of Dietary Partial Hydrolysate of Phospholipids, Rich in Docosahexaenoic Acid-Bound Lysophospholipids, on Lipid and Fatty Acid Composition in Rat Serum and Liver.
Effect of Dietary Partial Hydrolysate of Phospholipids, Rich in Docosahexaenoic Acid-Bound Lysophospholipids, on Lipid and Fatty Acid Composition in Rat Serum and Liver.
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DOI：
10.1111/1750-3841.14416
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发表时间：
2019-01
影响因子：
3.9
通讯作者：
Ryota Hosomi;K. Fukunaga;T. Nagao;Toshifumi Tanizaki;Kazumasa Miyauchi;Munehiro Yoshida;S. Kanda;Toshimasa Nishiyama;Koretarō Takahashi
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文献类型：
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作者：
Ryota Hosomi;K. Fukunaga;T. Nagao;Toshifumi Tanizaki;Kazumasa Miyauchi;Munehiro Yoshida;S. Kanda;Toshimasa Nishiyama;Koretarō Takahashi
溶血磷脂已被认为是有效的生物活性脂质介质。然而，尚未注意到膳食磷脂部分水解物（PH-PL）的健康益处，其富含二十二碳六烯酸（DHA）结合的溶血磷脂。在这项研究中，PH-PL对大鼠的血清和肝脏脂质分布的影响，PH-PL的管理后，证明了在比较的鱼油（FO），其中包括二十碳五烯酸（EPA）和DHA结合的甘油三酯（TG）。以富含磷脂的鱿鱼粉为原料，通过酶法改性制备了含有EPA和DHA的PH-PL。雄性Wistar大鼠饲喂仅含大豆油（7%）、FO和PH-PL的基础饲料。FO和PH-PL日粮中EPA和DHA的含量相似。在给大鼠喂食各自的饮食28 d后，测定其血清和肝脏脂质含量、粪便脂质排泄量和肝脏基因表达水平。结果表明，与单饲大豆油相比，PH-PL饲料降低了血清和肝脏TG含量，其部分原因是由于PH-PL饲料提高了肝脏酰基辅酶A氧化酶活性，抑制了肝脏脂肪酸合成酶活性。此外，与大豆油饮食相比，PH-PL组表现出较低的血清胆固醇含量，至少部分是因为肝脏3-羟基-3-甲基戊二酰辅酶A还原酶mRNA表达水平的降低。我们发现，膳食中添加含有PH-PL的EPA和DHA具有降血脂作用，可能有助于预防与生活方式相关的疾病。
Lysophospholipids have been recognized as potent biologically active lipid mediators. However, attention has not been paid to the health benefits of dietary partial hydrolysate of phospholipids (PH-PL), which is rich in docosahexaenoic acid (DHA)-bound lysophospholipids. In this study, the effects of PH-PL on serum and liver lipid profiles of rats upon administration of PH-PL are demonstrated in comparison to those of fish oil (FO), which comprises eicosapentaenoic acid (EPA) and DHA-bound triglyceride (TG). PH-PL containing EPA and DHA was prepared via enzymatic modification of squid (Todarodes pacificus) meal that is rich in phospholipids. Male Wistar rats were fed a basal diet containing soybean oil alone (7%), FO, and PH-PL. The FO and PH-PL diets had similar EPA and DHA contents. After the rats had been fed their respective diets for 28 d, their serum and liver lipid contents, fecal lipid excretion, and hepatic gene expression level were measured. The results demonstrated that compared with the soybean oil diet alone, the PH-PL diet decreased serum and liver TG contents partially because of the enhancement of liver acyl-CoA oxidase activity and suppression of liver fatty acid synthase activity. In addition, compared with the soybean oil diet, the PH-PL group exhibited lower serum cholesterol content at least in part because of the reduction of hepatic 3-hydroxy-3-methylglutaryl-CoA reductase mRNA expression level. We found that dietary administration of EPA and DHA containing PH-PL has a hypolipidemic effect that may help prevent the development lifestyle-related diseases.