Photoacoustic and fluorescence image-guided surgery using a multifunctional targeted nanoprobe.
Photoacoustic and fluorescence image-guided surgery using a multifunctional targeted nanoprobe.
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DOI：
10.1245/s10434-014-3541-9
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发表时间：
2014-05
影响因子：
3.7
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Xi L;Zhou G;Gao N;Yang L;Gonzalo DA;Hughes SJ;Jiang H
肿瘤切缘阴性的完整手术切除是预测大多数实体瘤癌症患者长期生存的最重要因素。我们假设使用纳米粒子增强的光声和荧光成像的图像引导手术可以显著降低局部复发率。使用侵袭性乳腺癌的小鼠模型。包括三个实验组：（1）对照;（2）注射非靶向纳米探针的荷瘤小鼠;和（3）注射靶向纳米探针的荷瘤小鼠。外科医生在光声成像（派）的引导下切除了原发性肿瘤，然后检查了手术伤口，并使用术中近红外（NIR）荧光成像切除了可疑组织。每周对小鼠进行生物发光成像以量化局部复发。纳米探针增强的光声对比度使派映射的体积肿瘤边缘高达31毫米的深度。有针对性的纳米粒子提供了显着更大的增强比非靶向纳米粒子。注射靶向纳米探针的组中的七只小鼠基于NIR荧光成像进行了额外的切除。病理学分析证实在5/7只小鼠的再切除标本中残留癌细胞。图像引导切除导致局部复发显著减少;靶向组和对照组中分别有8.7%和33.3%的小鼠复发。这些结果表明，光声和近红外术中成像可以有效地帮助外科医生定位原发性肿瘤，并实时识别残留疾病。该技术有望克服目前导致需要第二次手术的临床挑战。
A complete surgical excision with negative tumor margins is the single most important factor in the prediction of long-term survival for most cancer patients with solid tumors. We hypothesized that image-guided surgery using nanoparticle-enhanced photoacoustic and fluorescence imaging could significantly reduce the rate of local recurrence. A murine model of invasive mammary carcinoma was utilized. Three experimental groups were included: (1) control; (2) tumor-bearing mice injected with non-targeted nanoprobe; and (3) tumor-bearing mice injected with targeted nanoprobe. The surgeon removed the primary tumor following the guidance of photoacoustic imaging (PAI), then inspected the surgical wound and removed the suspicious tissue using intraoperative near-infrared (NIR) fluorescence imaging. The mice were followed with bioluminescence imaging weekly to quantify local recurrence. Nanoprobe-enhanced photoacoustic contrast enabled PAI to map the volumetric tumor margins up to a depth of 31 mm. The targeted nanoparticles provided significantly greater enhancement than non-targeted nano-particles. Seven mice in the group injected with the targeted nanoprobes underwent additional resections based upon NIR fluorescence imaging. Pathological analysis confirmed residual cancer cells in the re-resected specimens in 5/7 mice. Image-guided resection resulted in a significant reduction in local recurrence; 8.7 and 33.3 % of the mice in the targeted and control groups suffered recurrence, respectively. These results suggest that photoacoustic and NIR intraoperative imaging can effectively assist a surgeon to locate primary tumors and to identify residual disease in real-time. This technology has promise to overcome current clinical challenges that result in the need for second surgical procedures.