Pathogenic characteristics of a novel intranuclear coccidia in Japanese black calves and its genetic identification as Eimeria subspherica
Pathogenic characteristics of a novel intranuclear coccidia in Japanese black calves and its genetic identification as Eimeria subspherica
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DOI：
10.1007/s00436-017-5629-1
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发表时间：
2017-11-01
影响因子：
2
通讯作者：
Shibahara, Tomoyuki
中科院分区：
文献类型：
--
作者：
Koreeda, Terunori;Kawakami, Tomo;Shibahara, Tomoyuki
牛核内球虫病是由原生动物阿拉巴马艾美耳球虫和环孢子虫引起。在这里，我们对患有慢性和难治性水样腹泻的日本黑小牛的疾病进行了表征，并从基因上鉴定出了致病物种。组织学检查显示空肠绒毛萎缩，空肠上皮细胞核内有大量寄生虫。基于使用我们设计的18S核糖体RNA基因特异性引物的分子分析，发现该寄生虫在系统发育树中与亚球形艾美耳球虫形成在同一簇中，与其他相关的艾美耳球虫属分开。这些结果构成了亚球形埃球菌作为牛核内球虫病病因的首次报道。
Bovine intranuclear coccidiosis is caused by the protozoans Eimeria alabamensis and Cyclospora spp. Here, we characterized the disease and genetically identified the causative species in Japanese black calves with chronic and refractory watery diarrhea. Histologic examinations revealed atrophy of the jejunal villi and numerous parasites in the nucleus of epithelial cells in the jejunum. Based on molecular analyses using 18S ribosomal RNA gene-specific primers that we designed, the parasites were found to be formed in the same cluster as Eimeria subspherica in the phylogenetic tree, which was separated from those of other related Eimeria spp. These results constitute the first report of E. subspherica as a cause of bovine intranuclear coccidiosis.