Emissive europium complexes that stain the cell walls of healthy plant cells, pollen tubes and roots
Emissive europium complexes that stain the cell walls of healthy plant cells, pollen tubes and roots
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DOI：
10.1039/c3ra45426f
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发表时间：
2014-01
期刊：
影响因子：
3.9
通讯作者：
A. J. Palmer;S. Ford;Stephen J. Butler;Timothy J. Hawkins;P. Hussey;R. Pal;James W. Walton;D. Parker
中科院分区：
文献类型：
--
作者：
A. J. Palmer;S. Ford;Stephen J. Butler;Timothy J. Hawkins;P. Hussey;R. Pal;James W. Walton;D. Parker
在烟草BY-2细胞和花粉管、本氏烟草（Nicotiana benthamiana）植物叶和野生型植物拟南芥（哥伦比亚）的根毛中研究了一组五种发射性铕络合物的细胞染色行为。细胞壁的选择性染色，特别是在烟草BY-2细胞，由复合物[EuL 1]，其中包含一个azathiaxanthone发色团。内化只发生在那些被故意渗透或正在死亡的细胞中。在健康的植物叶片内没有观察到吸收。在根毛中，细胞壁以及线粒体被强烈染色，通过延时显微镜显示活组织中线粒体的翻滚，通过共定位研究证实。在花粉管中，细胞壁也被染色;在与[Eu.L1]孵育后，通过用405 nm激光激发触发，发生花粉尖端的快速爆裂。这种行为与细胞壁渗透性和完整性的局部扰动一致，与发色团三重激发态的反应性相关。
The cell-staining behaviour of a set of five emissive europium complexes has been studied in Nicotiana tabacum BY-2 cells and pollen tubes, Nicotiana benthamiana plant leaves and in the root hairs of the wild-type plant Arabidopsis thaliana (Columbia). The cell walls were stained selectively, notably in the tobacco BY-2 cells, by the complex [EuL1] that contains one azathiaxanthone chromophore. Internalisation only occurred in cells that had been deliberately permeabilised or were dying. No uptake was observed within healthy plant leaves. In root hairs, the cell wall was strongly stained as well as the mitochondria, revealed by time-lapsed microscopy that showed the tumbling of the mitochondria in the living tissue, confirmed by co-localisation studies. In pollen tubes, the cell wall was also stained; rapid bursting of the pollen tip occurred following incubation with [Eu.L1], triggered by excitation with 405 nm laser light. Such behaviour is consistent with local perturbation of cell wall permeability and integrity, associated with the reactivity of the chromophore triplet excited state.