Study on Inactivation Effects and Regeneration Inhibition Mechanism of Atmospheric-Pressure Helium Plasma Jet on Acinetobacter Baumannii Biofilm
Study on Inactivation Effects and Regeneration Inhibition Mechanism of Atmospheric-Pressure Helium Plasma Jet on Acinetobacter Baumannii Biofilm
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常压氦等离子体射流对鲍曼不动杆菌生物膜的灭活效果及再生抑制机制研究
DOI：
10.1109/tps.2020.3042809
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发表时间：
2021-01-01
影响因子：
1.5
通讯作者：
Cheng, Cheng
中科院分区：
文献类型：
--
作者：
Hu, Shuheng;Ye, Xiaodong;Cheng, Cheng
研究了常压氦脉冲放电等离子体射流对多药耐药鲍曼不动杆菌生物膜的灭活机制及对生物膜再生能力的影响。最初的细菌总人口是7.37 + / - 0.16日志(10)集落形成单位(CFU) /毫升在生物膜和大约99%的a . baumannii生物膜细胞失去cultivability 30分钟后血浆治疗。同时，reazurin荧光染色法显示，约80%的生物膜细胞失去代谢能力，部分细菌进入有活力但不可培养的状态。此外，在SYTO 9/碘化丙啶（PI）染色试验中，38.3%的细菌失去了膜的完整性。H(2)DCFDA荧光染色显示，暴露于血浆后细菌体内活性氧（ROS）浓度短暂升高后趋于初始状态。此外，在对鲍曼不饱和鲍曼杆菌在血浆暴露30min后再生能力的研究中，再生后的细菌总数为5.36 +/- 0.20 log(10) CFU/mL。与第一代生物膜中可培养细菌总数相比，约99.99%的细菌被抑制。相比之下，再生生物膜的生物量下降到（11.0 +/- 1.0）%。同时，代谢菌的比例降至（52.9 +/- 1.0）%。以上结果表明，常压氦等离子体射流不仅能有效灭活生物膜中的细菌，还能抑制下一代生物膜的形成。
The inactivation mechanism on multidrug-resistant Acinetobacter baumannii biofilms by atmospheric-pressure helium pulse discharge plasma jet and the effect on the biofilm regeneration capacity were studied. The initial total bacterial population was 7.37 +/- 0.16 log(10) colony forming unit (CFU)/mL in biofilms and about 99% of the A. baumannii biofilm cells lost their cultivability after 30 min of plasma treatment. Meanwhile, resazurin fluorescent staining method showed that about 80% of the biofilm cells lost their metabolic capacity, and some bacteria entered a viable but nonculturable state. In addition, 38.3% of the bacteria lost their membrane integrity in the light of SYTO 9/propidium iodide (PI) staining assay. According to H(2)DCFDA fluorescent staining, the concentration of reactive oxygen species (ROS) in bacteria after plasma exposure tended to initial state after a short increase. Moreover, in the study of the regeneration ability of A. baumannii after plasma exposure for 30 min, the total bacterial number after regeneration was 5.36 +/- 0.20 log(10) CFU/mL. Compared with the total number of cultivable bacteria in the first-generation biofilm, about 99.99% of the bacteria were inhibited. In comparison, the biomass of the regenerated biofilm decreased to (11.0 +/- 1.0)%. Meanwhile, the percentage of metabolic bacteria decreased to (52.9 +/- 1.0)%. These results indicated that atmospheric-pressure helium plasma jet could not only effectively inactivate bacteria in biofilms, but also inhibit the formation of the next generation biofilms.