DEMONSTRATION OF IMMUNOCHEMICAL IDENTITY BETWEEN THE SYNAPTIC VESICLE PROTEINS SYNAPTIN AND SYNAPTOPHYSIN P38
DEMONSTRATION OF IMMUNOCHEMICAL IDENTITY BETWEEN THE SYNAPTIC VESICLE PROTEINS SYNAPTIN AND SYNAPTOPHYSIN P38
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DOI：
10.1016/0014-5793(88)80997-9
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发表时间：
1988-12-19
期刊：
影响因子：
3.5
通讯作者：
BOCK, E
中科院分区：
文献类型：
--
作者：
GAARDSVOLL, H;OBENDORF, D;BOCK, E
突触囊泡蛋白Synaptin和Synaptophysin/p38在免疫化学上是相同的。免疫印迹分析表明，抗突触素的多克隆多特异性抗体和抗突触素的单抗SY38均可与38 kDa的条带反应。在牛肾上腺髓质嗜铬颗粒膜的二维免疫印迹中，抗突触素和抗突触素抗体也识别相同的成分。最后，在免疫印迹实验中，SY38与免疫分离的突触素抗原发生了反应。
The synaptic vesicle proteins synaptin and synaptophysin/p38 were shown to be immunochemically identical. Western immunoblot analysis of Triton X‐100 extracts from rat brain showed that polyclonal polyspecific anti‐synaptin antibodies and monoclonal antibody SY38 against synaptophysin both reacted with a band of 38 kDa. In two‐dimensional immunoblots of chromaffin granule membranes from bovine adrenal medulla anti‐synaptin and anti‐synaptophysin antibodies also recognized the same component. Finally, in a Western immunoblotting experiment SY38 reacted with an immunoisolated synaptin antigen.