Spontaneous depolarization wave in the mouse embryo: origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging.
Spontaneous depolarization wave in the mouse embryo: origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging.
复制标题
小鼠胚胎中的自发去极化波：通过电压敏感染料成像识别的中枢神经系统的起源和大规模传播。
DOI：
10.1111/j.1460-9568.2012.07997.x
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发表时间：
2012
影响因子：
3.4
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y.;Nakamori;T. and Sato;K.
胚胎自发运动是由脑干和脊髓网络驱动的颅神经和脊神经的相关自发活动产生的。利用电压敏感染料的光学成像，我们之前已经揭示了这种相关活动是一种广泛传播的神经去极化波，我们称之为去极化波。我们在小鸡和大鼠胚胎中观察到，这种活动遍布中枢神经系统的广泛区域，包括脊髓、后脑、小脑、中脑和前脑。一个重要的考虑是，具有类似特征的去极化波是否出现在其他物种中，特别是在不同的哺乳动物中。在这里，我们通过显示广泛传播的波独立于先前用Ca2+成像检测到的局部自发活动，为小鼠胚胎中去极化波的存在提供了证据。此外，我们绘制了去极化波的起源，并揭示了随着发育的进行，波发生器从吻侧脊髓移动到尾侧脊髓，后来被成熟的节律发生器所取代。本研究以及一篇描述小鼠去极化波药理特性的论文表明，具有共同特征的同步波在不同物种中表达，这表明在神经发育中起着重要作用。
Spontaneous embryonic movements, called embryonic motility, are produced by correlated spontaneous activity in the cranial and spinal nerves, which is driven by brainstem and spinal networks. Using optical imaging with a voltage‐sensitive dye, we have revealed previously that this correlated activity is a widely propagating wave of neural depolarization, which we termed the depolarization wave. We have observed in the chick and rat embryos that the activity spread over an extensive region of the CNS, including the spinal cord, hindbrain, cerebellum, midbrain and forebrain. One important consideration is whether a depolarization wave with similar characteristics occurs in other species, especially in different mammals. Here, we provide evidence for the existence of the depolarization wave in the mouse embryo by showing that the widely propagating wave appeared independently of the localized spontaneous activity detected previously with Ca2+imaging. Furthermore, we mapped the origin of the depolarization wave and revealed that the wave generator moved from the rostral spinal cord to the caudal cord as development proceeded, and was later replaced with mature rhythmogenerators. The present study, together with an accompanying paper that describes pharmacological properties of the mouse depolarization wave, shows that a synchronized wave with common characteristics is expressed in different species, suggesting fundamental roles in neural development.