A case series and immunophenotypic analysis of CK20-/CK7+primary neuroendocrine carcinoma of the skin
A case series and immunophenotypic analysis of CK20-/CK7+primary neuroendocrine carcinoma of the skin
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DOI：
10.1111/j.1600-0560.2007.00759.x
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发表时间：
2007-12-01
影响因子：
1.7
通讯作者：
Morgan, Michael B.
中科院分区：
文献类型：
--
作者：
Calder, Kenneth B.;Coplowitz, Shana;Morgan, Michael B.
背景资料：默克尔细胞癌（MCC）的诊断是相当具有挑战性的，因此，免疫组化的配置文件是重要的，在确认显微镜下诊断。MCC的神经内分泌和上皮分化的特征，细胞角蛋白（CK）20，CK 7，上皮膜抗原和神经元特异性烯醇化酶等抗体，用于确认诊断。如文献报道，大多数MCC表达CK 20，CK 7阴性。在此，我们提出了一个病例系列的7例CK 20-/CK 7+原发性皮肤神经内分泌carcinoma.Methods：所有病例MCC与特定CK 20-/CK 7+染色文件在一个大型退伍军人医院和三级转诊皮肤病理服务进行了审查。所有7例进行了分析的临床，病理和免疫表型属性。结果：所有病例均表现为CK 7弥漫性胞浆染色和突触素阳性染色。CK 20和甲状腺转录因子-1染色是negative.Conclusions：在此，我们提出了一个迄今未报道的CK 20-/CK 7+原发性皮肤神经内分泌癌的患者组。本病例系列的目的是描述MCC的一种新的免疫表型变异，同时进一步扩展了CK 20-/CK 7+染色肿瘤亚组中肿瘤的鉴别诊断。
Background: The diagnosis of Merkel cell carcinoma (MCC) can be rather challenging; therefore, the immunohistochemical profile is important in confirming the microscopic diagnosis. Characteristic of the neuroendocrine and epithelial differentiation of MCC, antibodies to cytokeratin (CK) 20, CK7, epithelial membrane antigen, and neuron-specific enolase among others, are used in confirming the diagnosis. As reported in the literature, the majority of MCC express CK20 and are CK7 negative. Herein, we present a case series of seven patients with CK20-/CK7+ primary cutaneous neuroendocrine carcinoma.Methods: All cases of MCC with specific CK20-/CK7+ staining on file at a large veterans' hospital and tertiary referral dermatopathology service were reviewed. All seven cases were analyzed for clinical, pathologic and immunophenotypic attributes. All specimens were submitted for immunohistochemical staining and interpreted by a single dermatopathologist.Results: All the cases showed diffuse cytoplasmic staining for CK7 and positive staining for synaptophysin. CK20 and thyroid transcription factor-1 staining was negative.Conclusions: Herein we have presented a hitherto unreported group of patients with CK20-/CK7+ primary neuroendocrine carcinoma of the skin. The purpose of this case series is to describe a new immunophenotypic variant of MCC, while further expanding the differential diagnosis of tumors included in the subset of neoplasms showing CK20-/CK7+ staining.