Demonstration of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms
Demonstration of Chlamydia pneumoniae in the walls of abdominal aortic aneurysms
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DOI：
10.1016/s0741-5214(97)70260-x
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发表时间：
1997-03-01
影响因子：
4.3
通讯作者：
Saikku, P
中科院分区：
文献类型：
--
作者：
Juvonen, JJ;Tuvonen, T;Saikku, P
背景：血清流行病学研究表明慢性肺炎衣原体感染与冠心病之间存在关联。该微生物是一种常见的呼吸道病原体，已在主动脉和冠状动脉的动脉粥样硬化病变中得到证实。腹主动脉瘤经常与动脉粥样硬化相关，炎症实际上可能是动脉瘤扩张的重要因素。因此可以推测，肺炎衣原体可能在维持炎症和引发主动脉瘤的发展中发挥作用。 方法和结果：通过免疫组织化学分析、聚合酶链反应放大omp1基因、透射电子显微镜和培养方法，以组织学动脉粥样硬化阴性的人主动脉组织作为对照组，检查腹主动脉瘤标本中是否存在肺炎衣原体。通过免疫组织化学，分别在 12 个动脉瘤标本中的 12 个和 8 个中发现了衣原体脂多糖和肺炎衣原体特异性抗原，并且在所研究的 6 个动脉瘤标本中的 6 个中可以证明肺炎衣原体 DNA。此外，电子显微镜显示在测试的四个动脉瘤标本中的三个中存在衣原体样基体。对照样品聚合酶链式反应均未呈阳性反应，均未检出肺炎衣原体抗原。结论：肺炎衣原体常见于腹主动脉瘤血管壁。肺炎衣原体在这些动脉瘤发展中的潜在致病作用仍有待研究。
Background: Seroepidemiologic studies have indicated an association between chronic Chlamydia pneumoniae infection and coronary heart disease. The organism, which is a common respiratory pathogen, has been demonstrated in atherosclerotic lesions of the aorta and coronary arteries. Abdominal aortic aneurysms are frequently associated with atherosclerosis, and inflammation may actually be an important factor in aneurysmal dilatation. Hence it could be assumed that C. pneumoniae may play a role in maintaining an inflammation and triggering the development of aortic aneurysms.Methods and Results: Specimens from abdominal aortic aneurysm were examined for the presence of C. pneumoniae by immunohistochemical analysis, the polymerase chain reaction amplifying omp1 gene, transmission electron microscopy, and culture methods with histologically atherosclerosis-negative human aortic tissues used as a control group. Chlamydial lipopolysaccharide and C. pneumoniae specific antigens were found by immunohistochemistry in 12 and 8 of 12 aneurysm specimens, respectively, and C. pneumoniae DNA could be demonstrated in 6 of 6 aneurysm specimens studied. Furthermore electron microscopy revealed the presence of Chlamydia-like elementary bodies in three of four aneurysm specimens tested. None of the control samples gave positive reaction in the polymerase chain reaction, and C. pneumoniae antigens were not detected in any of them.Conclusions: C. pneumoniae is frequently found in the vessel wall of abdominal aortic aneurysm. The potential etiopathogenetic role of C. pneumoniae in the development of these aneurysms remains to be studied.