Removal of patulin from apple juice using inactivated lactic acid bacteria
Removal of patulin from apple juice using inactivated lactic acid bacteria
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DOI：
10.1111/j.1365-2672.2012.05279.x
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发表时间：
2012-05-01
影响因子：
4
通讯作者：
Mohamad, O.
中科院分区：
文献类型：
--
作者：
Hatab, S.;Yue, T.;Mohamad, O.
目的：苹果和苹果产品是受棒曲霉(PAT)污染最明显的商品，对人体健康和经济造成不利影响。本研究的主要目的是考察10株不同的灭活乳酸菌(LAB)对苹果汁中PAT的去除效果。方法和结果：所有受试菌都能与47%~80%的PAT结合，而鼠李糖乳杆菌6224和粪肠球菌21605使PAT分别下降了80.4%和64.5%。结果表明，PAT的结合依赖于毒素的初始浓度和吸附温度，10株细菌的生物量也存在差异。红外光谱分析确定了与PAT吸附相关的潜在官能团和可能的结合部位。结论：PAT的去除是菌株特异性的。结果表明，生物吸附过程不影响果汁的品质。FTIR分析表明，细胞壁对PAT的吸附起着关键作用。研究的意义和影响：我们的结果证明，灭活的LAB具有有效结合PAT的潜力，是一种新型的、有前途的吸附剂。
Aims: Apples and apple products are the most notably commodities contaminated with patulin (PAT), which cause detrimental effects on human health and economic problems. The primary objective of this study was to investigate the removal of PAT contamination from apple juice using 10 different inactivated lactic acid bacteria (LAB) strains.Methods and Results: Significant quantities of PAT ranging from 47 to 80% were bound to all tested bacterial strains, whereas Lactobacillus rhamnosus 6224 and Enterococcus faecium 21605 caused a decrease of PAT by 80.4 and 64.5%, respectively. The results showed that the binding of PAT depends on the initial concentration of toxin and the adsorption temperature, also the differences in biomass existed among the 10 bacterial strains. IR analysis was performed to identify potential functional groups and the possible binding sites related to PAT adsorption.Conclusions: The removal of PAT was observed to be strain specific. The results indicated that the biosorption process did not affect the quality of juice. FTIR analysis showed that the cell wall plays a key role in PAT adsorption.Significance and Impact of the Study: Our results proof that inactivated LAB have the potential as a novel and promising adsorbent to bind PAT effectively.