Brief azacytidine step allows the conversion of suspension human fibroblasts into neural progenitor-like cells.
Brief azacytidine step allows the conversion of suspension human fibroblasts into neural progenitor-like cells.
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简短的azacytidine步骤允许将悬浮液的人成纤维细胞转化为神经祖细胞样细胞。
DOI：
10.22074/cellj.2015.522
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发表时间：
2015
期刊：
影响因子：
2
通讯作者：
Baharvand H
中科院分区：
文献类型：
--
作者：
Mirakhori F;Zeynali B;Kiani S;Baharvand H
近年来，转分化技术使人成纤维细胞的直接转化成为生物医学研究中患者特异性诱导细胞产生的有价值的、丰富的和可获得的细胞来源。大多数转分化方法依赖于病毒基因递送，其由于与宿主基因组的随机整合可在移植时引起基因组不稳定性和肿瘤发生。在这里，我们提供了一种简单的方法来诱导神经祖细胞样细胞从人成纤维细胞没有遗传操作，通过改变物理化学培养特性从单层培养到悬浮液中的化学DNA甲基转移酶抑制剂，氮杂胞苷的存在下。我们已经证明了神经祖细胞样标志物的表达，形态和自发分化为神经样细胞的能力。这种方法简单，廉价，缺乏遗传操作，可能是未来化学神经转分化和安全诱导神经祖细胞从人成纤维细胞用于临床应用的基础。
In recent years transdifferentiation technology has enabled direct conversion of human fibroblasts to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. The majority of transdifferentiation approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorigenesis upon transplantation. Here, we provide a simple way to induce neural progenitor-like cells from human fibroblasts without genetic manipulation by changing physicochemical culture properties from monolayer culture into a suspension in the presence of a chemical DNA methyltransferase inhibitor agent, Azacytidine. We have demonstrated the expression of neural progenitor-like markers, morphology and the ability to spontaneously differentiate into neural-like cells. This approach is simple, inexpensive, lacks genetic manipulation and could be a foundation for future chemical neural transdifferentiation and a safe induction of neural progenitor cells from human fibroblasts for clinical applications.