Bacteriophage lambda cro mutations: effects on activity and intracellular degradation.
Bacteriophage lambda cro mutations: effects on activity and intracellular degradation.
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噬菌体 lambda cro 突变：对活性和细胞内降解的影响。
DOI：
10.1073/pnas.83.23.8829
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发表时间：
1986
影响因子：
11.1
通讯作者：
Sauer,RT
中科院分区：
文献类型：
--
作者：
Pakula,AA;Young,VB;Sauer,RT
在对噬菌体lambda cro基因进行随机突变后，我们分离到了影响Cro 66个残基中大约一半的错义突变。大约三分之二的突变改变了与维持Cro结构和稳定性有关的残基。相应的突变蛋白在细胞内被严重降解，但通常具有接近野生型Cro的特定活性。其余的突变会影响与DNA结合有关的残基。这些突变蛋白存在于适度降低的细胞内水平，但它们的比活性远低于野生型。
Following random mutagenesis of the bacteriophage lambda cro gene, we have isolated missense mutations that affect approximately half of the 66 residue positions of Cro. About two-thirds of the mutations change residues involved in the maintenance of Cro structure and stability. The corresponding mutant proteins are severely degraded in the cell but often have specific activities near that of wild-type Cro. The remaining mutations affect residues involved in DNA binding. These mutant proteins are present at moderately reduced intracellular levels, but their specific activities are much lower than that of wild type.