Effect of human procathepsin D on proliferation of human cell lines.
Effect of human procathepsin D on proliferation of human cell lines.
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DOI：
10.1016/0304-3835(94)90251-8
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发表时间：
1994-05
期刊：
影响因子：
9.7
通讯作者：
V. Vetvicka;J. Vĕktvicková;M. Fusek
中科院分区：
文献类型：
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作者：
V. Vetvicka;J. Vĕktvicková;M. Fusek
我们已经使用从人乳腺癌细胞系ZR-75-1的上清液中分离的人组织蛋白原D来测试其对广谱人源细胞系的促有丝分裂活性。这些细胞系包括：乳腺癌细胞系ZR-75-1、MDA-MB-436、MBA-MD-483和MDA-MB-231、B淋巴母细胞样细胞系Raji、单核细胞样细胞系U937、T淋巴母细胞样细胞系8402、上皮样癌细胞系HELA、肝细胞癌细胞系Hep G2、母乳上皮细胞系HBL-100和血管肉瘤细胞系HAEND-1。我们已经测试了这些细胞系的增殖水平，这取决于培养基中组织蛋白酶原D的存在。同时，我们也测量了胰岛素样生长因子II在相同的实验条件下的效果。我们已经发现IGF II的影响与前组织蛋白酶D的影响之间存在显著差异。虽然IGF II以几乎相同的方式促进所有测试细胞系的增殖，但组织蛋白原D仅对乳腺癌细胞系具有非常明显的作用。这一发现可能有助于解释一些相互矛盾的结果，在人类乳腺癌的预后意义的组织蛋白原D。
We have used human procathepsin D isolated from supernatant of human breast cancer cell line ZR-75-1 to test its mitogenic activity for a broad spectrum of human-derived cell lines. These cell lines included: breast cancer cell lines ZR-75-1, MDA-MB-436, MBA-MD-483 and MDA-MB-231, B lymphoblastoid cell line Raji, the monocytoid cell line U937, T lymphoblastoid cell line 8402, epitheloid carcinoma cell line HELA, hepatocellular carcinoma cell line Hep G2, breast milk epithelial cell line HBL-100 and angiosarcoma cell line HAEND-1. We have tested the level of proliferation of these cell lines depending on the presence of procathepsin D in the medium. In parallel we have also measured the effect of insulin-like growth factor II under the same experimental conditions. We have found a significant difference between the influence of IGF II and that of procathepsin D. While IGF II promoted in practically the same way the proliferation of all cell lines tested, procathepsin D had a very pronounced effect on breast cancer cell lines only. This finding might help to explain some contradictory results of prognostic significance of procathepsin D in human breast cancer.