Differential regulation by exercise of BDNF and NT-3 in rat spinal cord and skeletal muscle
Differential regulation by exercise of BDNF and NT-3 in rat spinal cord and skeletal muscle
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DOI：
10.1046/j.0953-816x.2001.01484.x
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发表时间：
2001-03-01
影响因子：
3.4
通讯作者：
Edgerton, VR
中科院分区：
文献类型：
--
作者：
Gómez-Pinilla, F;Ying, Z;Edgerton, VR
我们研究了神经肌肉活动对成年大鼠腰段脊髓和支配骨骼肌中神经营养因子表达的影响。大鼠在跑台上运动1d或连续5d，在最后一次运动后0、2、6h处死动物。到第一天，没有明显的证据表明脊髓或比目鱼肌中脑源性神经营养因子(BDNF)的mRNA增加。到第5天，脊髓中BDNF mRNA在训练后2小时显著增加，比目鱼肌在训练后0~6小时表现出强劲的增加。免疫组织化学分析显示，在训练第5天，比目鱼肌中BDNF蛋白的表达显著增加。免疫组织化学分析显示，运动神经元胞体和腹角中的轴突中的BDNF水平升高。测定神经营养因子-3(NT-3)的mRNA，以确定所选择的神经营养因子是否对神经肌肉活动有选择性的诱导模式。在脊髓中，单次训练后脊髓中NT-3mRNA的表达呈进行性下降，而在训练后2 h至第5天，NT-3mRNA的表达显著增加。比目鱼肌中的NT-3mRNA在训练后第1天和第5天呈进行性增加。这些结果表明，神经肌肉活动对BDNF和NT-3系统有特定的影响，重复运动影响这些反应的大小和稳定性。
We have investigated the impact of neuromuscular activity on the expression of neurotrophins in the lumbar spinal cord region and innervating skeletal muscle of adult rats. Rats were exercised on a treadmill for 1 day or 5 consecutive days and euthanized at 0, 2 or 6 h after the last bout of exercise. By Day 1, there was no clear evidence of an increase in brain-derived neurotrophic factor (BDNF) mRNA in the spinal cord or the soleus muscle. By Day 5, there was a significant increase in BDNF mRNA in the spinal cord at 2 h post-training, and the soleus muscle showed a robust increase between 0 and 6 h post-training. Immunoassays showed significant increases in BDNF protein in the soleus muscle by training Day 5. Immunohistochemical analyses showed elevated BDNF levels in motoneuron cell bodies and axons in the ventral horn. Neurotrophin-3 (NT-3) mRNA was measured to determine whether selected neurotrophins respond with a selective pattern of induction to neuromuscular activity. In the spinal cord, there was a progressive post-training decrease in NT-3 mRNA following a single bout of training, while there was a significant increase in NT-3 mRNA at 2 h post-training by Day 5. The soleus muscle showed a progressive increase in NT-3 mRNA by Days 1 and 5 following training. These results show that neuromuscular activity has specific effects on the BDNF and NT-3 systems, and that repetitive exercise affects the magnitude and stability of these responses.