UniPrime: a workflow-based platform for improved universal primer design
UniPrime: a workflow-based platform for improved universal primer design
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DOI：
10.1093/nar/gkn191
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发表时间：
2008-06-01
影响因子：
14.9
通讯作者：
Teeling, Emma C.
中科院分区：
文献类型：
--
作者：
Bekaert, Michael;Teeling, Emma C.
UniPrime是一个开源软件（http://uni prime.batlab）。eu)，只需输入一个基因ID参考，就能自动设计大量的通用引物。UniPrime自动从GenBank中检索和比对同源序列，识别比对中的保守区域，并生成合适的引物来扩增可变基因组区域。UniPrime与以前的自动引物设计程序的不同之处在于引物设计的所有步骤都是自动化的，保存的，并且系统发育有限。通过对多个目哺乳动物的4个不同基因（AOF2、EFEMP1、LRP6和OAZ1）进行扩增和测序，实验验证了该程序的有效性和成功性。UniPrime是一个经过实验验证的全自动程序，可以生成考虑到PCR生物学方面的成功的跨物种引物。
UniPrime is an open-source software (http://uni prime.batlab.eu), which automatically designs large sets of universal primers by simply inputting a gene ID reference. UniPrime automatically retrieves and aligns homologous sequences from GenBank, identifies regions of conservation within the alignment and generates suitable primers that can amplify variable genomic regions. UniPrime differs from previous automatic primer design programs in that all steps of primer design are automated, saved and are phylogenetically limited. We have experimentally verified the efficiency and success of this program by amplifying and sequencing four diverse genes (AOF2, EFEMP1, LRP6 and OAZ1) across multiple Orders of mammals. UniPrime is an experimentally validated, fully automated program that generates successful cross-species primers that take into account the biological aspects of the PCR.