Distribution of steroid- and dioxin-like activities between sediments, POCIS and SPMD in a French river subject to mixed pressures
Distribution of steroid- and dioxin-like activities between sediments, POCIS and SPMD in a French river subject to mixed pressures
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DOI：
10.1007/s11356-012-1452-5
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发表时间：
2013-05-01
影响因子：
5.8
通讯作者：
Ait-Aissa, Selim
中科院分区：
文献类型：
--
作者：
Creusot, Nicolas;Tapie, Nathalie;Ait-Aissa, Selim
内分泌干扰化学物质（EDCs）对水生系统的污染现在是一个广泛确立的事实。然而，关于这些活性化合物的来源、转运、命运和生物利用度的知识仍然缺乏。在本研究中，我们使用极性有机化合物集成采样器（POCIS）和半透膜装置（SPMD）被动采样器评估了沉积物和水间的雌激素，（抗）雄激素，妊娠X受体样（PXR）和二恶英样活性的分布，该采样器先前被描述为沉积物高度和多重污染。我们首先确认了该河流沉积物在2004年、2009年和2010年的污染模式，表明该河流处于持续的高污染水平。然而，我们发现这些活性在不同区室之间的分布模式不同：雌激素活性主要在POCIS提取物中检测到，在沉积物和SPMD提取物中检测到的程度较小；SPMD和底泥提取物中主要检测到抗雄激素活性，而POCIS提取物中未检测到抗雄激素活性；在所有三个被调查的隔间中都检测到pxr样活性，其中POCIS > SPMD >沉积物；类二恶英活性主要存在于沉积物和SPMD提取物中。总体而言，生物活性的划分与每次生物测定中典型已知活性化学物质的物理化学性质（例如，log K (low)）一致。此外，为了确定参与这些活动的化学物质是否在隔室之间相似，我们使用多步骤分离程序对沉积物、POCIS和SPMD提取物进行了分离。这突出了车厢之间活性化学物质性质的差异。总之，我们的结果支持需要考虑不同的间隔，以加强暴露评估。
The contamination of aquatic systems by endocrine disrupting chemicals (EDCs) is now a widely established fact. Nevertheless, there is still a scarcity of knowledge concerning the source, transport, fate and bioavailability of such active compounds. In the present study we assessed the distribution of estrogenic, (anti-)androgenic, pregnane X receptor-like (PXR) and dioxin-like activities between sediment and water compartments using a polar organic compound integrative sampler (POCIS) and a semi-permeable membrane device (SPMD) passive sampler in a river where sediment has been previously described as highly and multi-contaminated. We first confirmed the contamination pattern of this river sediment between 2004, 2009 and 2010 samples, suggesting that this river is subject to a constant high contamination level. However, we showed a different distribution pattern of these activities between compartments: estrogenic activity was mainly detected in POCIS extracts and to a lesser extent in sediment and SPMD extracts; anti-androgenic activities were mainly detected in SPMD and sediment extracts while no activity was detected in POCIS extracts; PXR-like activity was detected in all three investigated compartments, with POCIS > SPMD > sediment; dioxin-like activity was mainly found in the sediment and the SPMD extracts. Overall, partitioning of the biological activities was in accordance with physicochemical properties (e.g., log K (ow)) of typical known active chemicals in each bioassay. Furthermore, in order to establish whether the chemicals involved in these activities were similar between the compartments, we fractionated sediment, POCIS and SPMD extracts using a multi-step fractionation procedure. This highlighted differences in the nature of active chemicals between compartments. Altogether, our results support the need to consider different compartments in order to enhance exposure assessment.