Impact of social dominance hierarchy on PACAP expression in the extended amygdala, corticosterone, and behavior in C57BL/6 male mice.
Impact of social dominance hierarchy on PACAP expression in the extended amygdala, corticosterone, and behavior in C57BL/6 male mice.
复制标题
社会支配等级对 C57BL/6 雄性小鼠杏仁核中 PACAP 表达、皮质酮和行为的影响。
DOI：
10.1101/2023.05.03.539254
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Bolshakov,VadimY
中科院分区：
文献类型：
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作者：
Meloni,EdwardG;CarlezonJr,WilliamA;Bolshakov,VadimY
动物与社会统治等级的自然结合会在大脑中产生适应性和潜在的不适应变化，从而影响健康和行为。动物通过优势相互作用而表现出的攻击性和顺从行为涉及到依赖压力的神经和激素系统，这些系统已被证明与社会地位相对应。在这里，我们研究了在集体饲养的实验室小鼠笼子中建立的社会统治等级对扩展杏仁核区域（包括终纹床核（BNST）和杏仁核中央核（CeA））中垂体腺苷酸环化酶激活肽（PACAP）表达的影响。我们还量化了优势等级对皮质酮（CORT）、体重和行为（包括旋转杆和声惊吓反应）的影响。体重匹配的雄性C57BL/6小鼠，从3周龄开始分组饲养（4只/笼），根据12周龄时在改变饲养条件后评估的攻击性和顺从性遭遇次数，将其分为最占优势（优势）、最不占优势（顺从）或中间等级（中间）。我们发现，与其他两组相比，顺从小鼠的BNST中PACAP的表达明显高于其他两组，而CeA中PACAP的表达则不高。在服从型小鼠中，CORT水平最低，这似乎反映了在社会优势互动后的迟钝反应。体重、运动协调性和声惊吓在各组间无显著差异。综上所述，这些数据揭示了在社会统治等级最低的动物中占优势的特定神经/神经内分泌系统的变化，并暗示PACAP在通过社会统治等级发展而发生的大脑适应中起作用。
The natural alignment of animals into social dominance hierarchies produces adaptive, and potentially maladaptive, changes in the brain that influence health and behavior. Aggressive and submissive behaviors assumed by animals through dominance interactions engage stress-dependent neural and hormonal systems that have been shown to correspond with social rank. Here, we examined the impact of social dominance hierarchies established within cages of group-housed laboratory mice on expression of the stress peptide pituitary adenylate cyclase-activating polypeptide (PACAP) in areas of the extended amygdala comprising the bed nucleus of the stria terminalis (BNST) and central nucleus of the amygdala (CeA). We also quantified the impact of dominance rank on corticosterone (CORT), body weight, and behavior including rotorod and acoustic startle response. Weight-matched male C57BL/6 mice, group-housed (4/cage) starting at 3 weeks of age, were ranked as either most-dominant (Dominant), least-dominant (Submissive) or in-between rank (Intermediate) based on counts of aggressive and submissive encounters assessed at 12 weeks-old following a change in homecage conditions. We found that PACAP expression was significantly higher in the BNST, but not the CeA, of Submissive mice compared to the other two groups. CORT levels were lowest in Submissive mice and appeared to reflect a blunted response following social dominance interactions. Body weight, motor coordination, and acoustic startle were not significantly different between the groups. Together, these data reveal changes in specific neural/neuroendocrine systems that are predominant in animals of lowest social dominance rank, and implicate PACAP in brain adaptations that occur through the development of social dominance hierarchies.