Simpler and faster quartz crystal microbalance for macromolecule detection using Fixed Frequency Drive
Simpler and faster quartz crystal microbalance for macromolecule detection using Fixed Frequency Drive
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DOI：
10.1016/j.snb.2022.131442
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发表时间：
2022-01
期刊：
影响因子：
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通讯作者：
A. Guha;N. Sandström;V. Ostanin;D. Klenerman;Sourav K. Ghosh
中科院分区：
文献类型：
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作者：
A. Guha;N. Sandström;V. Ostanin;D. Klenerman;Sourav K. Ghosh
尽管分析技术取得了进步，但其复杂性和成本在很大程度上限制了其在可扩展在线或多路测量中的应用。在这里，我们报告了一种基于石英晶体谐振器(QCR)的大分子检测方法，通过首次使用固定频率驱动器(FFD)和声学参数的解析表达式，可以极大地简化和快速测量。使用人免疫球蛋白E(HIGE)作为样本大分子和QCR上官能化的抗HIGE适配子，与传统的阻抗分析方法进行了定量准确性的基准测试。FFD能够在较长的观察期内以显著更高的采集速率在固定幅度下捕获数据，这表明QCR对转导的敏感性和特异性有所改善。文中还讨论了低成本、低功耗的在线集成和大规模可复用的基础。
Despite advancements in analytical technologies, their complexity and cost have largely restricted their application in scalable online or multiplexed measurements. Here we report a quartz crystal resonator (QCR)-based method for detection of macromolecules that allows immensely simpler and faster measurements by employing for the first time a fixed frequency drive (FFD) and analytical expressions of acoustic parameters. Using human immunoglobulin E (hIgE) as an exemplar macromolecule and an anti-hIgE aptamer functionalised on a QCR, quantitative accuracy was benchmarked against the traditional impedance analysis method. The ability of FFD to capture data over longer observation periods at significantly higher acquisition rates at a fixed amplitude showed improvement in the QCR’s sensitivity and specificity of transduction. The foundations for low-cost and low-power online integration and large-scale multiplexability are also discussed.