A novel, efficient method for otolith specimen preparation using UV‐cured resins
A novel, efficient method for otolith specimen preparation using UV‐cured resins
复制标题
使用紫外线固化树脂制备耳石标本的新颖、高效方法
DOI：
10.1002/nafm.10484
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发表时间：
2020
影响因子：
1.1
通讯作者：
Mitsui Shota
中科院分区：
文献类型：
--
作者：
Strussmann Carlos A.;Miyoshi Kaho;Mitsui Shota
与大多数脊椎动物一样，耳石是位于鱼类内耳的钙化结构，主要负责重力、平衡和运动的感知，其次负责声音检测。内耳石生长层的微观结构和化学分析（称为增量）构成了估计鱼类年龄的强大工具，并阐明了鱼类种群的许多生活史和人口特征。耳石分析通常需要生成薄横截面，其中包括耳石核心以及同一视图平面中从出生（核心）到收集时刻（耳石边缘）形成的所有增量。在这里，我们报告了单组分紫外线固化树脂获取耳石横截面的适用性，用于微观结构检查和通过电子探针微量分析进行化学分析。用紫外线固化树脂生产的嵌入块具有与常用的双组分环氧树脂相当的透明度，并且在使用精密切割机和分析进行研磨或切割期间为耳石提供类似的粘附力和机械支撑。此外，紫外线固化树脂可按需提供极其快速（分钟级）的硬化，这使得它们在耳石嵌入时间成为限制的课堂环境中非常有用。
Otoliths are calcified structures that are located in the inner ears of fish, as in most vertebrates, that are responsible primarily for the perception of gravity, balance, and movement and secondarily of sound detection. Microstructural and chemical analyses of the inner otolith growth layers, called increments, constitute powerful tools for estimating fish age and elucidate many life history and demographic traits of fish populations. Otolith analyses often require the production of a thin cross section that includes the otolith core and all increments formed from birth (core) until the moment of collection (otolith edge) in the same plane of view. Here we report on the suitability of single‐component UV‐cured resins to obtain otolith cross sections for both microstructural examination and chemical analysis by electron probe microanalysis. Embedding blocks that were produced with UV‐cured resins had transparency that was comparable to that of the commonly used two‐component epoxy resins and provided similar adhesion and mechanical support for the otoliths during grinding or cutting with a precision cutter and analysis. In addition, UV‐cured resins offer on‐demand, extremely rapid (minute‐order) hardening that makes them useful in classroom situations in which time for otolith embedding becomes a constraint.