Low salt medium for lactate and ethanol production by recombinant Escherichia coli B
Low salt medium for lactate and ethanol production by recombinant Escherichia coli B
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DOI：
10.1007/s10529-006-9252-y
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发表时间：
2007-03-01
影响因子：
2.7
通讯作者：
Ingram, L. O.
中科院分区：
文献类型：
--
作者：
Martinez, Alfredo;Grabar, T. B.;Ingram, L. O.
通过实验改变各个营养盐，以确定大肠杆菌 B 重组菌株有效生产 L(+)-乳酸的最低要求。根据这些结果，配制了低水平碱金属（4.5 mM 和总盐（4.2 g l(-1)）的 AM1 培养基。该培养基对于从木糖生产乙醇和从葡萄糖生产乳酸同样有效，平均生产率为 18-19 mmol l(-1) h(-1) 两者（发酵最初 48 小时）。
Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient L(+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l(-1)). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18-19 mmol l(-1) h(-1) for both (initial 48 h of fermentation).