Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-to-glucose conversion
Cellulase immobilized mesoporous silica nanocatalysts for efficient cellulose-to-glucose conversion
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DOI：
10.1039/c1gc15563f
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发表时间：
2011-01-01
期刊：
影响因子：
9.8
通讯作者：
Wu, Kevin C. -W.
中科院分区：
文献类型：
--
作者：
Chang, Renee Han-Yi;Jang, Jen;Wu, Kevin C. -W.
合成了两种具有不同粒径、孔径和表面积的介孔二氧化硅纳米颗粒（MSN），并将其用作固定纤维素酶的支架，纤维素酶是一种可以在水溶液中水解纤维素的酶。采用物理吸附和化学结合两种不同的固定方法来固定纤维素酶。对纤维素水解的反应条件，包括温度、时间和纤维素酶的用量进行了优化。本研究系统地研究了纤维素酶固定化MSN催化剂的负载量、催化活性和稳定性。结果表明，与具有羧基和大孔径的MSN化学连接的纤维素酶可以实现超过80%的产率和优异的稳定性，有效地将纤维素转化为葡萄糖。
Two mesoporous silica nanoparticles (MSNs) with different particle size, pore size, and surface area were synthesized and utilized as scaffolds for the immobilization of cellulase, an enzyme that can hydrolyze cellulose in aqueous solution. Two different immobilization methods, i.e., physical adsorption and chemical binding, were used to immobilize cellulase. The reaction conditions, including temperature, time, and the amount of cellulase for cellulosic hydrolysis, were optimized. This study systematically investigates the loading amount, catalytic activity, and stability of cellulase immobilized MSN catalysts. Results show that the cellulase chemically linked to MSN exhibiting carboxyl groups and a large pore size could achieve an effective cellulose-to-glucose conversion exceeding 80% yield and excellent stability.