Characterization and expression pattern of the novel MIA homolog TANGO
Characterization and expression pattern of the novel MIA homolog TANGO
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DOI：
10.1016/j.modgep.2003.12.002
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发表时间：
2004-07-01
影响因子：
1.2
通讯作者：
Buettner, R
中科院分区：
文献类型：
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作者：
Bosserhoff, AK;Moser, M;Buettner, R
通过基因库搜索，发现了编码黑色素瘤抑制活性(MIA)同源蛋白的人类新基因Tango。Tango与同源基因Mia、Otor(Fpd，MIA1)和MIA2共同定义了一个新的基因家族，这些基因具有重要的结构特征，在核苷酸和蛋白质水平上都具有显著的同源性，并且具有相似的基因组结构。四个成员的氨基酸同源性为34-45%，cDNA序列同源性为47-59%。除了一个疏水性的分泌信号序列外，Tango还编码一个103个氨基酸的成熟蛋白质。序列同源性证实了MIA、Otor和Mia2中也存在高度保守的SH3结构。因此，似乎存在许多具有SH3折叠样结构的胞外蛋白。有趣的是，原位杂交、RTPCR和Northern blots显示了非常广泛的Tango表达模式，而不是以前为MIA基因家族的其他成员确定的高度限制性的表达模式。唯一缺乏探戈表达的细胞是属于造血系统的细胞。在胚胎发育过程中和成年组织中都观察到了高水平的探戈表达。(C)2003爱思唯尔B.V.保留所有权利。
A novel human gene, TANGO, encoding a MIA ('melanoma inhibitory activity') homologous protein was identified by a gene bank search. TANGO, together with the homologous genes MIA, OTOR (FPD, MIAL) and MIA2 define a novel gene family sharing important structural features, significant homology at both the nucleotide and protein level, and similar genomic organization. The four members share 34-45% amino acid identity and 47-59% cDNA sequence identity. TANGO encodes a mature protein of 103 amino acids in addition to a hydrophobic secretory signal sequence. Sequence homology confirms the highly conserved SH3 structure present also in MIA, OTOR and MIA2. Thus, it appears that there are a number of extracellular proteins with SH3-fold like structures. Interestingly, in situ hybridization, RTPCR and Northern Blots revealed very broad TANGO expression patterns in contrast to the highly restricted expression patterns previously determined for the other members of the MIA gene family. The only cells lacking TANGO expression are cells belonging to the hematopoetic system. High levels of TANGO expression were observed both during embryogenesis and in adult tissues. (C) 2003 Elsevier B.V. All rights reserved.