INFLUENCE OF LYSINE-VASOPRESSIN DOSAGE ON TIME COURSE OF CHANGES IN RENAL TISSUE AND URINARY COMPOSITION IN CONSCIOUS RAT
INFLUENCE OF LYSINE-VASOPRESSIN DOSAGE ON TIME COURSE OF CHANGES IN RENAL TISSUE AND URINARY COMPOSITION IN CONSCIOUS RAT
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DOI：
10.1113/jphysiol.1971.sp009383
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发表时间：
1971-01-01
影响因子：
5.5
通讯作者：
THOMAS, S
中科院分区：
文献类型：
--
作者：
ATHERTON, JC;GREEN, R;THOMAS, S
1.连续静脉输注赖氨酸加压素（2·5、5、15和60 μ u./至少100 g体重）在水负荷的清醒大鼠中持续长达4½小时。就尿液和组织成分而言，达到最大和稳定反应的幅度和所需时间均随剂量而变化。髓质成分的剂量依赖性变化包括含水量的分级降低和溶质（主要是钠和尿素）含量的分级增加。水、钠和尿素含量变化的相对贡献随时间和剂量而变化。所有剂量组的乳头尿素含量均显著增加。尿素含量的变化范围比其它溶质的变化范围大.在低剂量下，尿流量和渗透压摩尔浓度的变化几乎完全归因于游离水清除率的大幅降低，以及髓质成分的微小变化;在较高剂量下，尿渗透压摩尔浓度的增加伴随着髓质溶质浓度的急剧增加。在髓质Na浓度增加阶段发生可变的、剂量依赖性的、短暂的尿钠排泄;尿钠排泄峰值出现在乳头和尿液中最大渗透压和Na浓度的时间之前。乳头和尿液中渗透压、尿素和钠浓度的差异也随时间而变化。从肾内机制的角度讨论了赖氨酸加压素诱导的过渡态和稳态变化。可以得出结论，基于肾脏中存在几个激素敏感位点，每个位点具有单独的剂量反应和动力学特征，对数据进行了最合理的解释。
1. The changes in urinary and renal tissue composition induced by continuous, intravenous infusion of lysine‐vasopressin (2·5, 5, 15 and 60 μ‐u./min. 100 g body wt.) for up to 4½ hr in water‐loaded, conscious rats were determined.2. Both the magnitude of, and the time required to attain, maximal and stable responses, in respect to both urinary and tissue composition, varied with the dose.3. The dose‐dependent changes in medullary composition were compounded of graded decreases in water content and graded increases in solute (mainly Na and urea) content.4. The relative contribution of the changes in water, Na and urea contents varied with time and with dose. Significant increases in papillary urea content occurred with all doses. The range of change in urea content was wider than that for any other solute.5. At low doses, the changes in urinary flow and osmolality were ascribable, almost entirely, to large decreases in free‐water clearance, with minor changes in medullary composition; at higher doses, the increases in urinary osmolality were accompanied by steep increases in medullary solute concentrations.6. A variable, dose‐dependent, transient natriuresis occurred during the phase of increasing medullary Na concentration; the peak natriuresis preceded the times of maximal osmolal and Na concentrations in the papilla and urine.7. The differences in osmolal, urea and Na concentrations between papilla and urine also changed with time.8. Both the transitional and steady‐state changes induced by lysine‐vasopressin are discussed in terms of intrarenal mechanisms. It is concluded that the data are most reasonably interpreted on the basis that several hormone‐sensitive loci exist in the kidneys, each with individual dose—response and kinetic characteristics.