Evaluation of neurotoxicity of artificial dura mater and poly(lactic-glycolic acidcaprolactone) co-oligomer containing dibutyltin or tin (II) octylate in rats
Evaluation of neurotoxicity of artificial dura mater and poly(lactic-glycolic acidcaprolactone) co-oligomer containing dibutyltin or tin (II) octylate in rats
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人工硬脑膜和含有二丁基锡或辛酸锡(II)的聚乳酸-乙醇酸己内酯共聚低聚物对大鼠的神经毒性评价
DOI：
10.11299/brte.24.153
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Y. Aizawa
中科院分区：
文献类型：
--
作者：
Masayoshi Tsuji;M. Tsunoda;C. Sugaya;Y. Inoue;Sachiko Hirano;H. Katagiri;H. Akita;M. Saji;T. Yuba;T. Fukushima;T. Tsuchiya;Y. Aizawa
目的：合成可生物降解的人工硬脑膜(DM)具有预防病毒感染等优点。这种人工DM的主要成分是聚(乳酸-乙醇酸-己内酯)共低聚物(PLGC)。对于DM的合成，使用含有二丁基锡(DBT)和辛酸锡(OT)的催化剂。DBT和OT仍留在DM中。为了建立一种评价DM安全性的模型，将每个直径为1 cm的圆盘植入大鼠颅骨，观察其最大大小、神经毒性作用及含100ppm DBT或200ppm OT的PLGC(DBT-PLGC)或OT-PLGC(OT-PLGC)。将人工DM、DBTPLGC或OT-PLGC植入每只大鼠脑表面(DM、DBT-PLGC和OT-PLGC组)。对照组为假手术组。植入4周后，进行脉冲前抑制(PPI)和开场试验。用高效液相色谱法测定不同脑区的神经递质含量。结果：几只大鼠的脑组织均有严重损伤。在PPI或野外测试中，各组之间的任何指标都没有显著差异。DM组下丘脑多巴胺的平均值显著高于其他组。糖尿病组大鼠中脑5-HIAA/5-羟色胺的平均值明显高于对照组。结论：在目前的治疗方案下，植入后DM的影响较小，但由于植入后很可能会造成严重的损害，因此需要进一步的研究。作为一个实验模型，如果切割出直径为8 mm的圆形颅骨盘，可能会在对脑损伤较小的情况下获得更好的效果。
Objectives: A synthetic biodegradable artificial dura mater (DM) can provide benefits such as helping to avoiding viral infections. The major component of this artificial DM is poly (lactic-glycolic acid-caprolactone) co-oligomer (PLGC). For synthesis of the DM, a catalyst containing dibutyltin (DBT) and tin (II) octylate (OT) is used. DBT and OT remain in the DM. To establish a model for the evaluation of the safety of the DM, after intracranial implantation of each 1-cm-diameter disk, the maximum size for rats, the neurotoxic effects of the DM and a PLGC containing 100 ppm DBT (DBT-PLGC) or 200 ppm OT (OT-PLGC) were evaluated.Methods: A circular disk of cranial bone of 1 cm in diameter was cut from each rat's skull. The artificial DM, DBTPLGC, or OT-PLGC was implanted onto the surface of the brain of each rat (the DM, DBT-PLGC, and OT-PLGC groups). The control group underwent a sham operation. Four weeks after the implantation, prepulse inhibition (PPI) and open field tests were performed. The neurotransmitters in discrete brain regions were determined by highperformance liquid chromatography.Results: There was severe damage to the brains of several rats. There were no significant differences among the groups in any indexes in the PPI or open field tests. The mean value of dopamine in the hypothalamus in the DM group was significantly higher compared to that in the other groups. The mean 5-HIAA/5-HT in the midbrain of the DM group was significantly higher than that in the control group.Conclusion: Although the effects of DM after the implantation were slight under the current protocol, further studies are required because of the severe damage most likely induced by the implantation. As an experimental model, if the circular cranial bone disk cut out were 8 mm in diameter, it may provide better results with less damage to the brain.
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二丁基锡和大量锡的聚-L-丙交酯对突变星形胶质细胞系的毒性较高，而合成人工硬脑膜的聚-L-丙交酯的毒性较低
影响因子：
0.3
作者：
Tsuji;M.;Tsunoda.M.;et al.
通讯作者：
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DOI：
--
发表时间：
2012
期刊：
The Kitasato Medical Journal
影响因子：
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作者：
Tsunoda;M.;Ikeuchi;R.;Tsuji;M.;Inoue;Y.;Ito;K.;Katagiri;H.;Akita;H.;Saji;M.;Yuba;T.;Yamada;T.;Tsuchiya;T. and Aizawa;Y
通讯作者：
Y
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DOI：
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发表时间：
2006
期刊：
J774.1. Biomedical Research on Trace Elements 17(4)
影响因子：
--
作者：
Tsunoda;M.;Yamamoto;K.;Ito;K.;Inoue;Y.;Miki;T.;Kudo;Y.;Satoh;T. and Aizawa;Y.
通讯作者：
Y.
二氯化二丁基锡 (DBT) 抑制鼠巨噬细胞系 J774.1 中细胞因子的产生。
DOI：
--
发表时间：
2006
期刊：
Biomedical Research on Trace Elements 17・4
影响因子：
--
作者：
Tsunoda;M.;Yamamoto;K.;Ito;K.;Inoue;Y.;Miki;T.;Kudo;Y.;Satoh;T.;Aizawa;Y.
通讯作者：
Y.