In Vivo Real-Time Imaging of Extracellular Vesicles in Liver Regeneration via Aggregation-Induced Emission Luminogens
In Vivo Real-Time Imaging of Extracellular Vesicles in Liver Regeneration via Aggregation-Induced Emission Luminogens
复制标题
通过聚集诱导发射发光原对肝脏再生中的细胞外囊泡进行体内实时成像
DOI：
10.1021/acsnano.8b09776
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发表时间：
2019-03-01
期刊：
影响因子：
17.1
通讯作者：
Wang, Yuebing
中科院分区：
文献类型：
--
作者：
Cao, Hongmei;Yue, Zhiwei;Wang, Yuebing
细胞外小泡(EVS)通过转运货物来调节细胞间的通讯和许多病理生理事件，因此在肝脏病理学研究中备受关注。监测和了解这些EV的体内命运和治疗能力对于基于EV的诊断和治疗的发展和优化至关重要。在这里，我们展示了使用聚集诱导发射发光剂DPA-SCP来实时跟踪来自人胎盘来源的间充质干细胞(MSCs)来源的EV及其在小鼠急性肝损伤(ALI)模型中的治疗效果。在体外，DPA-SCP不会改变MSC来源的EVS的固有特性，并且显示出极低的毒性。此外，DPA-SCP比最流行的商业电动汽车跟踪器PKH26和DII具有更高的标记效率和跟踪能力。在体内，DPA-SCP在不影响其再生能力和治疗效果的情况下，准确、定量地跟踪了EVS在小鼠ALI模型中的7天行为。EVS的治疗作用可能与其减少炎性细胞浸润、提高细胞存活率和抗细胞凋亡作用有关。总之，具有AIE特征的DPA-SCP可作为在体实时成像肝再生EVS的有利且安全的示踪剂。
Extracellular vesicles (EVs) attract much attention in liver pathology because they regulate cell-cell communication and many pathophysiological events by transferring their cargos. Monitoring and understanding the in vivo fate and therapeutic capacity of these EVs is critical for the development and optimization of EV-based diagnosis and therapy. Herein, we demonstrate the use of an aggregation-induced emission luminogen, DPA-SCP, for the real-time tracking of EVs derived from human placenta-derived mesenchymal stem cells (MSCs) and their therapeutic effects in a mouse acute liver injury (ALI) model. In vitro, DPA-SCP does not alter the inherent characteristics of MSC-derived EVs and shows extremely low toxicity. Moreover, DPA-SCP exhibited superior labeling efficiency and tracking capability to the most popular commercial EV trackers, PKH26 and DiI. In vivo, DPA-SCP precisely and quantitatively tracked the behaviors of EVs for 7 days in the mouse ALI model without influencing their regenerative capacity and therapeutic efficacy. The therapeutic effects of EVs may attribute to their ability for reducing inflammatory cell infiltration, enhancing cell survival and antiapoptotic effects. In conclusion, DPA-SCP with an AIE signature serves as a favorable and safe tracker for in vivo real-time imaging of EVs in liver regeneration.