Phase-Sensitive Intracellular Doppler Fluctuation Spectroscopy
Phase-Sensitive Intracellular Doppler Fluctuation Spectroscopy
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DOI：
10.1103/physrevapplied.15.024043
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发表时间：
2021-02-18
影响因子：
4.6
通讯作者：
Nolte, David D.
中科院分区：
文献类型：
--
作者：
Choi, Honggu;Jeong, Kwan;Nolte, David D.
活组织或活组织检查中三维细胞内运动的动态光散射由于宽带多普勒频移而产生动态散斑，该频移被记录为从散斑波动光谱获得的宽带光谱。主动细胞内运动产生的多普勒频移从 10 mHz 到 10 Hz 跨越三个数量级。生物动力成像是一种利用低相干数字全息术执行的全视场光学相干断层扫描技术。全息重建允许相位恢复，但机械稳定性限制有利于零差检测而不是外差检测，因为零差对相位漂移相对不敏感。然而，通过从连续帧获得相移分布，相位偏移形成稳健的概率分布。相位移分布的相位概率密度函数捕获随时间变化的平均相位移，并用于重建稳定的外差谱。相敏生物动力学成像用于检测活体组织的 Levy 稳定分布，这可能反映细胞内介质内的重尾异常运输。
Dynamic light scattering from three-dimensional intracellular motions in living tissues or biopsies creates dynamic speckle due to broadband Doppler shifts that are recorded as broadband spectra obtained from speckle fluctuation spectroscopy. Doppler frequency shifts from active intracellular motion extend across three orders of magnitude from 10 mHz to 10 Hz. Biodynamic imaging is a full-field optical coherence tomography technique performed with low-coherence digital holography. The holographic reconstruction allows phase recovery, but mechanical stability limitations favor homodyne detection over heterodyne detection because homodyne is relatively insensitive to phase drift. However, by obtaining phase-displacement distributions from consecutive frames, the phase excursions form a robust probability distribution. The phase-probability density function of the phase-displacement distribution captures average phase displacements over time and is used to reconstruct stable heterodyne spectra. Phase-sensitive biodynamic imaging is applied to detect Levy-stable distributions from living tissue that may reflect heavy-tailed anomalous transport within intracellular media.