Bone marrow-derived mesenchymal stem cell and simvastatin treatment leads to improved functional recovery and modified c-Fos expression levels in the brain following ischemic stroke
Bone marrow-derived mesenchymal stem cell and simvastatin treatment leads to improved functional recovery and modified c-Fos expression levels in the brain following ischemic stroke
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DOI：
10.22038/ijbms.2018.29382.7100
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发表时间：
2018-10-01
影响因子：
2.2
通讯作者：
Charish, Jason
中科院分区：
文献类型：
--
作者：
Jahromi, Gila Pirzad;Shabanzadeh, Alireza P.;Charish, Jason
目的：骨髓间充质干细胞（BMSC）治疗中风后功能恢复的有益结果已经得到了很好的证实。此外，5-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶抑制剂也已显示出增加神经元存活并促进BMSC向炎症部位移动。然而，精确的机制介导的改善神经功能恢复在斯托克模型后，辛伐他汀和BMSCs的组合治疗仍然知之甚少。材料和方法：在这里，栓塞中风模型被用来实验诱导局灶性缺血性脑损伤，通过插入一个预先形成的凝块到大脑中动脉（MCA）。结果：脑缺血后7 d，辛伐他汀联合BMSCs可显著促进BMSCs迁移、内源性神经发生、动脉形成和星形胶质细胞活化，同时与单独BMSCs治疗相比，可减轻神经元损伤（P
Objective(s): The beneficial outcomes of bone marrow-derived mesenchymal stem cell (BMSC) treatment on functional recovery following stroke has been well established. Furthermore, 5-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors have also been shown to increase neuronal survival and promote the movement of BMSCs towards the sites of inflammation. However, the precise mechanisms mediating the improved neurological functional recovery in stoke models following a combination treatment of Simvastatin and BMSCs still remained poorly understood.Materials and Methods: Here, an embolic stroke model was used to experimentally induce a focal ischemic brain injury by inserting a preformed clot into the middle cerebral artery (MCA). Following stroke, animals were treated either with an intraperitoneal injection of Simvastatin, an intravenous injection of 3 x106 BMSCs, or a combination of these two treatments.Results: Seven days after ischemia, the combination of Simvastatin and BMSCs led to a significant increase in BMSC relocation, endogenous neurogenesis, arteriogenesis and astrocyte activation while also reducing neuronal damage when compared to BMSC treatment alone (P