Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy
Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy
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DOI：
10.1128/jb.00045-07
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发表时间：
2007-06-01
影响因子：
3.2
通讯作者：
Lidstrom, Mary E.
中科院分区：
文献类型：
--
作者：
Chistoserdova, Ludmila;Lapidus, Alla;Lidstrom, Mary E.
沿着甲烷、甲醇和甲基化胺代表重要的生物源大气成分;因此，不仅甲烷氧化菌而且非甲烷氧化甲基氧化菌在全球碳循环中发挥重要作用。对模式专性甲醇和甲胺利用者鞭毛甲基杆菌（菌株KT）的全基因组进行测序。基因组由约3 Mbp的单个环状染色体表示，可能编码总共2，766种蛋白质。基于基因组分析以及来自先前遗传和突变分析的结果，甲基营养通过甲醇和甲胺脱氢酶及其特定电子传递链组分、四氢甲氨蝶呤连接的甲醛氧化途径和同化和异化核酮糖单磷酸循环以及甲酸脱氢酶实现。一些甲基营养基因存在于更多的。一个（相同或不相同）副本。对单碳化合物的专性依赖似乎是由于不完全的三羧酸循环，因为没有潜在编码α-酮戊二酸、苹果酸或琥珀酸脱氢酶的基因是可识别的。分枝杆菌基因组在甲基营养功能方面，将鞭毛菌与先前测序的三种甲基营养菌的基因组进行了比较，所述三种甲基营养菌是扭脱甲基杆菌（一种α-变形杆菌，7 Mbp）、嗜石油甲基菌（一种β-变形杆菌，4 Mbp）和荚膜甲基球菌（一种γ-变形杆菌，3.3 Mbp）。引人注目的是，代谢和/或遗传上，甲基营养功能在M。鞭毛虫与M. capsulatus和M. extorquens的比在更密切相关的M. petroleiphilum物种，提供了第一个基因组证据的多系起源的甲基化Betaproteobacteria。
Along with methane, methanol and methylated amines represent important biogenic atmospheric constituents; thus, not only methanotrophs but also nonmethanotrophic methylotrophs play a significant role in global carbon cycling. The complete genome of a model obligate methanol and methylamine utilizer, Methylobaciflus flagellatus (strain KT) was sequenced. The genome is represented by a single circular chromosome of approximately 3 Mbp, potentially encoding a total of 2,766 proteins. Based on genome analysis as well as the results from previous genetic and mutational analyses, methylotrophy is enabled by methanol and methylamine dehydrogenases and their specific electron transport chain components, the tetrahydromethanopterin-linked formaldehyde oxidation pathway and the assimilatory and dissimilatory ribulose monophosphate cycles, and by a formate dehydrogenase. Some of the methylotrophy genes are present in more. than one (identical or nonidentical) copy. The obligate dependence on single-carbon compounds appears to be due to the incomplete tricarboxylic acid cycle, as no genes potentially encoding alpha-ketoglutarate, malate, or succinate dehydrogenases are identifiable. The genome of M. flagellatus was compared in terms of methylotrophy functions to the previously sequenced genomes of three methylotrophs, Methylobacterium extorquens (an alphaproteobacterium, 7 Mbp), Methylibium petroleiphilum (a betaproteobacterium, 4 Mbp), and Methylococcus capsulatus (a gamma-proteobacterium, 3.3 Mbp). Strikingly, metabolically and/or phylogenetically, the methylotrophy functions in M. flagellatus were more similar to those in M. capsulatus and M. extorquens than to the ones in the more closely related M. petroleiphilum species, providing the first genomic evidence for the polyphyletic origin of methylotrophy in Betaproteobacteria.