Pattern recognition analysis of proton nuclear magnetic resonance spectra of brain tissue extracts from rats anesthetized with propofol or isoflurane.
Pattern recognition analysis of proton nuclear magnetic resonance spectra of brain tissue extracts from rats anesthetized with propofol or isoflurane.
复制标题
DOI：
10.1371/journal.pone.0011172
复制
发表时间：
2010-06-17
期刊：
影响因子：
3.7
通讯作者：
Sakamoto A
中科院分区：
文献类型：
--
作者：
Kawaguchi H;Hirakawa K;Miyauchi K;Koike K;Ohno Y;Sakamoto A
全身麻醉是一种常规的外科手术，其安全性已得到临床结果的认可；然而，其在分子水平上的作用尚未阐明。全身麻醉药会影响大脑中的葡萄糖代谢。然而，麻醉药对除与葡萄糖有关的脑代谢物以外的其他代谢物的影响尚未得到很好的表征。我们使用质子核磁共振波谱的模式识别分析来可视化广泛使用的静脉麻醉剂异丙酚和挥发性麻醉剂异氟醚对整体脑代谢表型的变化。将大鼠随机分为5组(n = ，每组7只)。异丙酚和异氟醚分别麻醉2或6h，对照组不麻醉。麻醉后直接取出脑组织。从切除的整个大脑中提取亲水性化合物，并用质子核磁共振波谱进行测量。所有光谱数据均用主成分分析进行处理和分析，以比较代谢物的分布情况。通过绘制主成分(PC)分数将数据可视化。在地块中，每个点代表一个单独的样本。异丙酚组和异氟醚组在曲线图上分别聚集，当比较6小时组时，这种分离尤其明显。异丙酚组的PC评分与对照组有明显差异，尤其是6h组，而异氟醚组和对照组的PC评分差异较小。本研究结果表明异丙酚和异氟醚在麻醉状态下对整体脑代谢有不同程度的影响。
General anesthesia is routinely used as a surgical procedure and its safety has been endorsed by clinical outcomes; however, its effects at the molecular level have not been elucidated. General anesthetics influence glucose metabolism in the brain. However, the effects of anesthetics on brain metabolites other than those related to glucose have not been well characterized. We used a pattern recognition analysis of proton nuclear magnetic resonance spectra to visualize the changes in holistic brain metabolic phenotypes in response to the widely used intravenous anesthetic propofol and the volatile anesthetic isoflurane. Rats were randomized into five groups (n = 7 each group). Propofol and isoflurane were administered to two groups each, for 2 or 6 h. The control group received no anesthesia. Brains were removed directly after anesthesia. Hydrophilic compounds were extracted from excised whole brains and measured by proton nuclear magnetic resonance spectroscopy. All spectral data were processed and analyzed by principal component analysis for comparison of the metabolite profiles. Data were visualized by plotting principal component (PC) scores. In the plots, each point represents an individual sample. The propofol and isoflurane groups were clustered separately on the plots, and this separation was especially pronounced when comparing the 6-h groups. The PC scores of the propofol group were clearly distinct from those of the control group, particularly in the 6-h group, whereas the difference in PC scores was more subtle in the isoflurane group and control groups. The results of the present study showed that propofol and isoflurane exerted differential effects on holistic brain metabolism under anesthesia.
登录
查看更多内容
影响因子：
8.8
作者：
Nguyen HT;Li KY;daGraca RL;Delphin E;Xiong M;Ye JH
通讯作者：
Ye JH
影响因子：
8.8
作者：
Alkire, MT
通讯作者：
Alkire, MT
影响因子：
2.9
作者：
Kobayashi, Katsuya;Takemori, Ken;Sakamoto, Atsuhiro
通讯作者：
Sakamoto, Atsuhiro
影响因子：
2.1
作者：
Oshima, T;Karasawa, F;Satoh, T
通讯作者：
Satoh, T
影响因子：
1.8
作者：
Nicholson, JK;Lindon, JC;Holmes, E
通讯作者：
Holmes, E