Elimination of creatine interference with the indophenol measurement of NH3 produced during nitrogenase assays.
Elimination of creatine interference with the indophenol measurement of NH3 produced during nitrogenase assays.
复制标题
消除肌酸对固氮酶测定过程中产生的 NH3 靛酚测量的干扰。
DOI：
10.1006/abio.1997.2517
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Fisher,K
中科院分区：
文献类型：
--
作者：
Dilworth,MJ;Fisher,K
结论。上述方法描述了一种获得活细菌细胞内 RNA 或 RNP 直接结构信息的有效、简单和方便的程序。它提供了有关鸟苷可及性的直接信息，无需对修饰位点进行费力的化学裂解，从而扩展和补充了现有的体内结构探测方法。我们已成功地将其应用于野生型和突变细胞内核糖体的结构比较，并且它应该普遍适用，而无需对各种结构研究进行深入的调整。致谢。我们感谢 Sabina Przibilla 测试了该方法的日常实验室适用性。这项工作得到了德国研究协会和化学工业基金会的支持。
Conclusions. The above method describes an efficient, easy, and convenient procedure for obtaining direct structural information on RNA or RNPs within living bacterial cells. It provides direct information on the accessibility of guanosines without laborious chemical cleavage of modified sites, and thereby extends and supplements existing methods for in vivo structural probing. We have successfully applied it for a structural comparison of ribosomes within wild-type and mutant cells, and it should be universally applicable without intensive adaptation to a wide variety of structural investigations.Acknowledgments. We thank Sabina Przibilla who tested the everyday lab fitness of the method. The work was supported by the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie.