Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter.
Organization, structure and alternate splicing of the murine RFC-1 gene encoding a folate transporter.
复制标题
编码叶酸转运蛋白的鼠 RFC-1 基因的组织、结构和可变剪接。
DOI：
10.1016/s0378-1119(96)00676-2
复制
发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
Sirotnak,FM
中科院分区：
文献类型：
--
作者：
Tolner,B;Roy,K;Sirotnak,FM
已经确定了编码叶酸转运蛋白的鼠RFC-1基因的结构组织。L1210细胞RFC-1cDNA全长、3′-和5′-非翻译区及编码序列分布于8个外显子，包括6个主要外显子和外显子1、5的交替外显子，全长10.4kb。在L1210细胞cDNA文库中鉴定剪接变体。最常见的包括外显子1至6，编码58 kDa多肽。两个最不常见的包括外显子1和2，外显子3的截短版本和外显子4至6;或外显子1至4，外显子5的替代，和外显子6，分别编码53.6和43.4 kDa的多肽。第四种变体（GenBank/EMBL登录号L36539）由其他人早先报道，它包含了我们发现的外显子1和外显子2至6的交替。外显子1的5′端以及外显子1a的基因组中GC相对丰富的区域似乎是明显的启动子样的，并编码许多推定的顺式作用元件。关于外显子1的交替的发现表明RFC-1变体的转录由两个不同的启动子引起。© 1997 Elsevier Science B. V.保留所有权利。
The structural organization of the murine RFC-1 gene encoding a folate transporter has been determined. The entire nucleotide sequence of the L1210 cell RFC-1 cDNA, the 3′- and 5′-untranslated regions and the coding sequence were found to be distributed in eight exons, including six primary exons and alternates to exon 1 and exon 5, spanning 10.4 kb. Splice variants were identified in an L1210 cell cDNA library. The most common incorporates exons 1 through 6, encoding a 58-kDa polypeptide. The two least common incorporate exons 1 and 2, a truncated version of exon 3 and exons 4 through 6; or exons 1 through 4, an alternate to exon 5, and exon 6, encoding polypeptides of 53.6 and 43.4 kDa, respectively. A fourth variant reported earlier (GenBank/EMBL accession No. L36539) by others incorporates what we have found to be an alternate of exon 1 and exons 2 through 6. A relatively GC-rich region of the genome just 5′ of exon 1 as well as exon 1a appears to be distinctly promoter-like and encodes a number of putative cis-acting elements. The findings pertaining to alternates of exon 1 suggest that the transcription of RFC-1 variants results from two different promoters. © 1997 Elsevier Science B.V. All rights reserved.