Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by Brain‐Derived Neurotrophic Factor
Regulation of Neuropeptide Expression in Cultured Cerebral Cortical Neurons by Brain‐Derived Neurotrophic Factor
复制标题
脑源性神经营养因子对培养的大脑皮层神经元中神经肽表达的调节
DOI：
10.1111/j.1471-4159.1993.tb03216.x
复制
发表时间：
1993
影响因子：
4.7
通讯作者：
K. Mizuno
中科院分区：
文献类型：
--
作者：
H. Nawa;Y. Bessho;J. Carnahan;S. Nakanishi;K. Mizuno
摘要：在培养的大脑皮层神经元上检测了神经营养因子的神经肽诱导活性。脑源性神经营养因子(BDNF)能显著增加神经肽生长抑素(SOM)和神经肽Y(NPY)的含量，但对八肽缩胆囊素(CCK)和GABA的影响不明显。20 ng/mlBDNF对NPY含量的诱导作用最强，为对照组的15倍。这些变化也在mRNA水平上重现。相反，神经营养素-3在增加NPY和SOM含量方面的作用要弱得多，神经生长因子对它们没有影响。NPY和SOM的mRNA表达完全依赖于BDNF的存在，但与BDNF的促存活活性无关，这一点已有报道。在培养的皮质神经元中，BDNF诱导的NPY免疫反应主要与谷氨酸脱羧酶免疫反应共存。这些结果提示，BDNF对大脑皮层GABA能神经元的肽能表达有调节作用。
Abstract: The neuropeptide‐inducing activity of neurotrophic factors was tested in cultured cerebral cortical neurons. Brain‐derived neurotrophic factor (BDNF) specifically increased contents of the neuropeptides somatostatin (SOM) and neuropeptide Y (NPY), but its effect on contents of cholecystokinin octapeptide and GABA was much less significant. The maximal induction of NPY content (15‐fold increase) was achieved by 20 ng/ml of BDNF. These changes were also reproduced at the mRNA level. In contrast, neurotrophin‐3 was much less potent at increasing NPY and SOM contents, and nerve growth factor had no effect on them. The expression of mRNA for NPY and SOM was fully dependent on the presence of BDNF in culture but irrelevant to the survival‐promoting activity of BDNF, which has been reported previously. Most of the NPY immunoreactivity induced by BDNF was colocalized with glutamate decarboxylase immunoreactivity in cultured cortical neurons. These results suggest that BDNF regulates the peptidergic expression of GABAergic neurons in the cerebral cortex.
睫状神经营养因子（CNTF）和去极化对培养交感神经元神经肽表达的影响。
影响因子：
2.7
作者：
Rao,MS;Tyrrell,S;Landis,SC;Patterson,PH
通讯作者：
Patterson,PH
DOI：
10.1073/pnas.88.3.961
发表时间：
1991-02-01
影响因子：
11.1
作者：
KNUSEL, B;WINSLOW, JW;HEFTI, F
通讯作者：
HEFTI, F