Androgen receptor gene expression in human prostate carcinoma cell lines.
Androgen receptor gene expression in human prostate carcinoma cell lines.
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发表时间：
1990-09
期刊：
影响因子：
11.2
通讯作者：
Wayne D. Tilley;C. M. Wilson;Marco Marcelli;M. McPhaul
中科院分区：
文献类型：
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作者：
Wayne D. Tilley;C. M. Wilson;Marco Marcelli;M. McPhaul
前列腺癌和前列腺癌细胞系对雄激素的反应差异很大。我们通过检测一个表达雄激素受体的前列腺癌细胞系(LNCaP)和两个不含可检测到雄激素受体的前列腺癌细胞系中雄激素受体的表达水平，探索了这种异质性的基础。我们发现，虽然LNCaP细胞株同时含有高水平的雄激素受体蛋白和mRNA，但受体阴性的细胞株DU-145和PC-3不表达雄激素受体蛋白，而通过Northern分析或S1核酸酶保护检测到不表达雄激素受体蛋白。这些结果表明，雄激素受体阴性细胞系中雄激素受体表达缺失是由于雄激素受体基因表达水平降低所致。基因组Southern分析表明，这些细胞系中雄激素受体表达的差异与雄激素受体基因结构的可检测变化无关。
Responses to androgen vary widely among prostate cancers and prostatic carcinoma cell lines. We have explored the basis for this heterogeneity by examining the levels of androgen receptor expression in a prostate carcinoma cell line (LNCaP) that expresses the androgen receptor and two prostate carcinoma cell lines that do not contain detectable androgen receptor. We find that while the LNCaP cell line contains high levels of both the androgen receptor protein and mRNA, the receptor-negative cell lines DU-145 and PC-3 do not express androgen receptor protein as detected by immunoblotting or mRNA as detected by Northern analysis or S1 nuclease protection. These results indicate that the absence of androgen receptor expression in the androgen receptor-negative cell lines is caused by diminished androgen receptor mRNA levels. Genomic Southern analysis indicates that the differences in androgen receptor expression in each of these cell lines is not associated with detectable alterations in the structure of the androgen receptor gene.