Nucleic acid-functionalized Pt nanoparticles: Catalytic labels for the amplified electrochemical detection of biomolecules
Nucleic acid-functionalized Pt nanoparticles: Catalytic labels for the amplified electrochemical detection of biomolecules
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DOI：
10.1021/ac0519864
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发表时间：
2006-04-01
影响因子：
7.4
通讯作者：
Willner, I
中科院分区：
文献类型：
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作者：
Polsky, R;Gill, R;Willner, I
核酸功能化的铂纳米颗粒（Pt-NPs）作为催化标记物用于DNA杂交和适体/蛋白质识别的放大电化学检测。核酸修饰的Pt-NP与传感核酸/分析物DNA复合物的杂交与电极相关联，使得能够通过Pt NP电催化还原H2 O2（灵敏度极限，1 × 10（-11）M）进行DNA的安培、放大检测。类似地，将适体官能化的Pt-NP与凝血酶适体/凝血酶复合物结合，该凝血酶复合物与电极结合，允许放大的、电催化的凝血酶检测，其灵敏度极限对应于1 × 10（-9）M。
Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 x 10(-11) M). Similarly, the association of aptamer-functionalized Pt-NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 x 10(-9) M.