Culturing microglia from the neonatal and adult central nervous system.
Culturing microglia from the neonatal and adult central nervous system.
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DOI：
10.3791/50647
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发表时间：
2013-08-09
期刊：
影响因子：
--
通讯作者：
Tsirka SE
中科院分区：
文献类型：
--
作者：
Bronstein R;Torres L;Nissen JC;Tsirka SE
小胶质细胞是中枢神经系统(CNS)内的巨噬细胞样细胞，在中枢神经系统发育成熟、多发性硬化症和脊髓损伤等生理和病理过程中具有重要作用。小胶质细胞可以通过神经元损伤或刺激来激活和招募活动，例如多发性硬化症的轴突损伤或中风引起的缺血性脑损伤。这些具有免疫活性的中枢神经系统成员也被认为在非病理条件下对突触的可塑性起作用。我们采用从新生儿和成人组织中培养小胶质细胞的方案，旨在最大限度地增加活细胞数量，同时最大限度地减少混杂变量，如其他中枢神经系统细胞类型和细胞培养碎片的存在。我们利用大的和容易辨别的中枢神经系统成分(例如皮质、脊髓节段)，这使得整个过程可行和可重复性。成年细胞的使用是使用新生儿脑小胶质细胞的合适替代方案，因为许多研究的病理学主要影响出生后的脊髓。这些培养系统也有助于直接测试可能抑制或促进小胶质细胞激活的化合物的效果。由于小胶质细胞的激活可以影响成人中枢神经系统疾病的转归，因此需要体外培养和研究新生儿和成人的小胶质细胞。
Microglia are the resident macrophage-like cells of the central nervous system (CNS) and, as such, have critically important roles in physiological and pathological processes such as CNS maturation in development, multiple sclerosis, and spinal cord injury. Microglia can be activated and recruited to action by neuronal injury or stimulation, such as axonal damage seen in MS or ischemic brain trauma resulting from stroke. These immunocompetent members of the CNS are also thought to have roles in synaptic plasticity under non-pathological conditions. We employ protocols for culturing microglia from the neonatal and adult tissues that are aimed to maximize the viable cell numbers while minimizing confounding variables, such as the presence of other CNS cell types and cell culture debris. We utilize large and easily discernable CNS components (e.g. cortex, spinal cord segments), which makes the entire process feasible and reproducible. The use of adult cells is a suitable alternative to the use of neonatal brain microglia, as many pathologies studied mainly affect the postnatal spinal cord. These culture systems are also useful for directly testing the effect of compounds that may either inhibit or promote microglial activation. Since microglial activation can shape the outcomes of disease in the adult CNS, there is a need for in vitro systems in which neonatal and adult microglia can be cultured and studied.
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作者：
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通讯作者：
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通讯作者：
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