HUMAN SPLEEN AS REVEALED BY SCANNING ELECTRON-MICROSCOPY
HUMAN SPLEEN AS REVEALED BY SCANNING ELECTRON-MICROSCOPY
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DOI：
10.1002/ajh.2830010209
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发表时间：
1976-01-01
影响因子：
12.8
通讯作者：
LUSHER, JM
中科院分区：
文献类型：
--
作者：
BARNHART, MI;LUSHER, JM
用扫描电子显微镜对动脉灌流和非灌流标本以及脾血管的塑料腐蚀模型进行扫描，以探索脾的三维世界。在研究的25个脾中，有18个是脾功能亢进的例子。这些脾与7个基本正常的儿童脾进行了对比，这些脾是为治疗霍奇金S病而分期的，他们的脾被证明没有参与病理过程。25个脾的脾窦均有不同程度的空洞。红细胞[红细胞]在从脾索进入鼻窦的过程中被捕获。这些窦窗代表开放循环通路的一端。在微血管铸型中，显示了直接的动静脉连接，为经常有争议的闭合循环通路奠定了解剖学基础。7例遗传性球形红细胞增多症患者的脾内可见超丰富的红髓。外周血红细胞上也可见脾索增厚并挤满球状细胞，许多球状细胞的膜稍有皱纹。这些膜特征可能是HS所特有的，反映了膜蛋白组成的固有异常。慢性特发性血小板减少性紫癜的7个脾以白髓为主。生发中心较多。微绒毛发达的淋巴细胞和浆细胞提示白髓可能存在抗血小板抗体的释放。外周白髓和边缘地带的血小板尤其明显。可见血小板聚集，通常与脾巨噬细胞相邻。
The 3-dimensional world of the spleen was explored by scanning EM on both arterially perfused and nonperfused specimens, as well as on plastic corrosion casts of splenic vasculatures. Of 25 spleens studied, 18 were examples of hypersplenism. These were contrasted to 7 essentially normal spleens taken from children being staged for treatment of Hodgkin''s disease whose spleens proved to be uninvolved in the pathologic process. Splenic sinuses in all 25 spleens were typified by a degree of porosity. RBC [red blood cells] were caught in the act of entering sinuses from splenic cords. These sinus windows represented one end of an open circulation pathway. In casts of microvasculature, direct arteriovenous connections were demonstrated, establishing an anatomical basis for an often disputed closed circulation pathway. Spleens from 7 patients with hereditary spherocytosis had a super abundance of red pulp. Splenic cords were thickened and crowded with spherocytes, many of which presented slightly wrinkled membranes, as were also noted on the peripheral blood RBC. It is possible that these membrane features were unique for HS and reflected the intrinsic membrane abnormality in protein composition. The 7 spleens from chronic idiopathic thrombocytopenic purpura had white pulp as the predominant region. Germinal centers were frequent. Lymphocytes and plasma cells with well-developed microvilli were suggestive that release of antiplatelet antibody might be occurring in white pulp. Platelets were especially notable in peripheral white pulp and marginal zones. Platelet clumps were observed, generally adjacent to splenic macrophages.