Comparative analysis of the effects of radiotherapy versus radiotherapy after adjuvant chemotherapy on the composition of lymphocyte subpopulations in breast cancer patients
Comparative analysis of the effects of radiotherapy versus radiotherapy after adjuvant chemotherapy on the composition of lymphocyte subpopulations in breast cancer patients
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DOI：
10.1016/j.radonc.2015.11.016
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发表时间：
2016-01-01
影响因子：
5.7
通讯作者：
Multhoff, Gabriele
中科院分区：
文献类型：
--
作者：
Sage, Eva K.;Schmid, Thomas E.;Multhoff, Gabriele
背景资料：乳腺癌是世界范围内女性最常见的癌症，手术、放疗（RT）和化疗（ChT）经常用于治疗这种癌症。辅助RT已显示引起外周血中淋巴细胞计数的长期变化。在此，在有和没有辅助ChT的患者中研究了RT后淋巴细胞亚群变化的时间过程，以探讨其潜在的临床影响。材料和方法：RT前（t0）、30戈伊后（t1）、RT结束时（t2）、6周（t3）、6个月（t4）、结果：40例乳腺癌患者放疗前和放疗后1年的淋巴细胞绝对计数均明显低于健康对照组，放疗后1年的淋巴细胞绝对计数明显低于放疗前和放疗后1年的对照组。在未接受ChT辅助治疗的患者中，CD 3（+）/CD 4（+）辅助性T细胞和FoxP 3（+）调节性T细胞的百分比显著增加。不同的NK细胞亚群下降，在RT与不ChT的患者，但恢复到初始水平RT后6个月（t4）。在RT（t0-t2）期间，未接受ChT治疗的患者CD 19（+）B细胞百分比显著下降，而接受辅助ChT治疗的患者CD 19（+）B细胞百分比逐渐升高。两组患者在RT后6个月（t4）达到初始水平。结论：不同的淋巴细胞亚群对有和无辅助ChT的RT反应不同。RT期间CD 4（+）T细胞增加，而NK细胞和B细胞减少，但在RT后6个月内恢复。Treg细胞在未接受ChT的患者中从t0到t5逐渐增加，但在接受辅助ChT的患者中没有。(C)2015爱思唯尔爱尔兰有限公司版权所有。
Background: Breast cancer is the most common cancer in women worldwide and surgery, radiotherapy (RT) and chemotherapy (ChT) are frequently used to treat this cancer. Adjuvant RT has been shown to cause long-term changes in lymphocyte counts in the peripheral blood. Herein, the time course of changes in lymphocyte subpopulations upon RT was studied in patients with and without adjuvant ChT in order to explore its potential clinical impact.Materials and methods: Total lymphocyte counts and the composition of lymphocyte subpopulations before RT (t0), after 30 Gy (t1), at the end of RT (t2), and 6 weeks (t3), 6 months (t4), and 1 year (t5) after RT were studied by flow cytometry.Results: Absolute lymphocyte counts were significantly lower in all breast cancer patients (n = 40) before and also 1 year after RT compared to healthy controls. The percentage of CD3(+)/CD4(+) helper T cells and FoxP3(+) regulatory T cells increased significantly in patients without adjuvant ChT. Different NK cell sub-populations dropped during RT in patients with and without ChT, but recovered to initial levels 6 months after RT (t4). During RT (t0-t2) the percentage of CD19(+) B cells significantly dropped in patients without ChT, but gradually increased in patients with adjuvant ChT. Both patient groups reached initial levels 6 months after RT (t4).Conclusion: Different lymphocyte subpopulations respond differently to RT with and without adjuvant ChT. CD4(+) T cells increase during RT, whereas NK cells and B cells decrease in patients without ChT, but recover within 6 months after RT. Treg cells gradually increase in patients without ChT from t0 to t5, but not in patients with adjuvant ChT. (C) 2015 Elsevier Ireland Ltd. All rights reserved.