Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
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DOI：
10.14411/eje.2009.056
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发表时间：
2009-01-01
影响因子：
1.3
通讯作者：
Spasic, Mihajlo B.
中科院分区：
文献类型：
--
作者：
Kojic, Danijela;Spasojevic, Ivan;Spasic, Mihajlo B.
本研究的目的是探讨抗氧化酶和活性氧的产生之间的关系滞育幼虫的欧洲玉米螟，欧洲玉米螟（鳞翅目：螟蛾科）保持在5 ℃，-3 ℃和-16 ℃两个星期。对该虫的过氧化氢（H_2O_2）含量、抗氧化酶、铜锌超氧化物歧化酶（CuZnSOD）、锰超氧化物歧化酶（MnSOD）和过氧化氢酶（CAT）的活性以及电子顺磁共振（EPR）谱进行了分析。在-3 ℃条件下，黑色素自由基含量高于5 ℃和-16 ℃，而CuZnSOD和CAT活性低于5 ℃和-16 ℃。在相同的温度（-3 ℃）下，记录到升高的H2 O2浓度。H2 O2在-3摄氏度，这是一个可能的调节作用的温度，触发抗冻性，建议。
The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.