Collagen I-Matrigel Scaffolds for Enhanced Schwann Cell Survival and Control of Three-Dimensional Cell Morphology
Collagen I-Matrigel Scaffolds for Enhanced Schwann Cell Survival and Control of Three-Dimensional Cell Morphology
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DOI：
10.1089/ten.tea.2008.0406
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发表时间：
2009-10-01
影响因子：
4.1
通讯作者：
Stegemann, Jan P.
中科院分区：
文献类型：
--
作者：
Dewitt, Daniel D.;Kaszuba, Stephanie N.;Stegemann, Jan P.
我们报告的能力，以控制三维许旺细胞（SC）的形态，使用胶原蛋白I-基质胶复合支架神经工程应用。SC支持损伤后的神经再生，最近有报道称，包埋在胶原蛋白I（一种常用于引导通道研究的材料）中的SC不容易延伸突起，而是采用指示与基质几乎没有相互作用的球形形态。我们已经修改了胶原蛋白I基质中加入基质胶，使他们更支持SC和这些矩阵和SC的形态特征在体外。掺入10%、20%、35%和50%体积的基质胶导致培养14天后平均SC过程长度比仅胶原I对照长2.4、3.5、3.7和4.2倍。此外，只有35%和50%的基质胶构建体能够在14天内维持SC数量，而在相同的时间段内，在仅胶原蛋白的构建体中观察到细胞从初始接种密度减少88%。机械测试显示，添加50%基质胶使基质硬度从仅胶原I结构中的6.4kPa增加到9.8kPa。此外，二次谐波产生成像显示，基质胶的加入导致I型胶原蛋白的不均匀分布，扫描电子显微镜成像显示了不同构建体的纤维结构的明显差异。总的来说，这项工作为开发同时支持神经元和SC用于未来神经工程应用的支架材料奠定了基础。
We report on the ability to control three-dimensional Schwann cell (SC) morphology using collagen I-Matrigel composite scaffolds for neural engineering applications. SCs are supportive of nerve regeneration after injury, and it has recently been reported that SCs embedded in collagen I, a material frequently used in guidance channel studies, do not readily extend processes, instead adopting a spherical morphology indicative of little interaction with the matrix. We have modified collagen I matrices by adding Matrigel to make them more supportive of SCs and characterized these matrices and SC morphology in vitro. Incorporation of 10%, 20%, 35%, and 50% Matrigel by volume resulted in 2.4, 3.5, 3.7, and 4.2 times longer average SC process length after 14 days in culture than with collagen I-only controls. Additionally, only 35% and 50% Matrigel constructs were able to maintain SC number over 14 days, whereas an 88% decrease in cells from initial seeding density was observed in collagen-only constructs over the same time period. Mechanical testing revealed that the addition of 50% Matrigel increased matrix stiffness from 6.4 kPa in collagen I-only constructs to 9.8 kPa. Furthermore, second harmonic generation imaging showed that the addition of Matrigel resulted in non-uniform distribution of collagen I, and scanning electron microscope imaging illustrated distinct differences in the fibrillar structure of the different constructs. Collectively, this work lays a foundation for developing scaffolding materials that are concurrently supportive of neurons and SCs for future neural engineering applications.