Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator
Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator
复制标题
DOI：
10.1016/s0969-2126(02)00761-x
复制
发表时间：
2002-05-01
期刊：
影响因子：
5.7
通讯作者：
Coll, M
中科院分区：
文献类型：
--
作者：
Blanco, AG;Sola, M;Coll, M
PhoB是一种信号转导反应调节因子，在磷酸盐耗竭条件下激活近40个基因。大肠杆菌和密切相关的细菌。与其靶DNA序列或pho盒复合的PhoB效应结构域的结构揭示了一种新的串联排列，其中几个单体将头到尾结合到连续的11个碱基对直接重复序列，覆盖平滑弯曲的双螺旋的一面。该蛋白质具有带翼的螺旋折叠，其中DNA识别元件包括穿透大沟的螺旋α 3和通过Arg219与压缩的小沟相互作用的β发夹翼，紧密夹在DNA糖骨架之间。反式激活环以适当的方向横向突出以与RNA聚合酶σ（70）亚基相互作用，其触发转录起始。
PhoB is a signal transduction response regulator that activates nearly 40 genes in phosphate depletion conditions in E. coli and closely related bacteria. The structure of the PhoB effector domain in complex with its target DNA sequence, or pho box, reveals a novel tandem arrangement in which several monomers bind head to tail to successive 11-base pair direct-repeat sequences, coating one face of a smoothly bent double helix. The protein has a winged helix fold in which the DNA recognition elements comprise helix alpha3, penetrating the major groove, and a beta hairpin wing interacting with a compressed minor groove via Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation loops protrude laterally in an appropriate orientation to interact with the RNA polymerase sigma(70) subunit, which triggers transcription initiation.