Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: absorption and circular dichroism studies
Subpicosecond UV spectroscopy of carbonmonoxy-myoglobin: absorption and circular dichroism studies
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DOI：
10.1039/b616173a
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Hache, Francois
中科院分区：
文献类型：
--
作者：
Dartigalongue, Thibault;Niezborala, Claire;Hache, Francois
利用亚皮秒可见光泵浦、紫外探测实验对一氧化碳肌红蛋白的吸收光谱和圆二色性进行了研究。在220-360 nm范围内的差分吸收表明，时间分辨的响应主要来自血红素和芳香族氨基酸没有显着贡献。在260 nm处的时间分辨CD显示没有动力学，并证实了这一结果。相反，在230 nm处观察到较强的CD动力学。该信号可能来源于蛋白质中α-螺旋的瞬时变形。
A thorough absorption and circular dichroism study is performed in carbonmonoxy-myoglobin with a sub-picosecond visible pump, ultraviolet probe experiment. Differential absorption in the 220-360 nm range shows that the time-resolved response mainly comes from the heme and that aromatic amino acids do not contribute significantly. Time-resolved CD at 260 nm shows no dynamics and confirms this result. On the contrary, a strong CD dynamics is observed at 230 nm. This signal could originate from transient deformation of the alpha-helices in the protein.