Endothelin-1 activates phospholipase C and mobilizes Ca2+ from extra- and intracellular pools in osteoblastic cells.
Endothelin-1 activates phospholipase C and mobilizes Ca2+ from extra- and intracellular pools in osteoblastic cells.
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Endothelin-1 激活磷脂酶 C 并从成骨细胞的细胞外和细胞内池中动员 Ca2。
DOI：
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发表时间：
1989
影响因子：
--
通讯作者：
Kamejiro Yamashita
中科院分区：
文献类型：
--
作者：
Y. Takuwa;Y. Ohue;N. Takuwa;Kamejiro Yamashita
本文研究了内皮素-1（ET）对成骨细胞MC 3 T3-E1的作用。在MC 3 T3-E1细胞中，ET特异性结合单一类别的高亲和力受体，并诱导磷脂酶C活化，产生两种第二信使，三磷酸肌醇和1，2-二酰基甘油，以及细胞内游离Ca 2+浓度（[Ca 2 +]i）的双相增加，其由初始瞬时增加和随后的持续平台组成，如用荧光指示剂fura-2测量的。清除细胞外Ca ~（2+）可消除ET诱导的第二平台期[Ca ~（2+）]i的短暂升高，但尼卡地平、维拉帕米或地尔硫卓均不能消除。ET-刺激的生产肌醇三磷酸没有取消细胞外Ca 2+的去除，表明ET-刺激的磷脂酶C激活是不是一个Ca 2+跨质膜流入增加的后果。ET刺激MC 3 T3-E1细胞DNA合成，降低碱性磷酸酶活性。蛋白激酶C激活剂佛波醇12，13-二丁酸酯模拟ET的这些作用。结果表明，ET激活肌醇脂质信号通路，并诱导从细胞外和细胞内池的Ca 2+的动员和成骨细胞MC 3 T3-E1细胞中蛋白激酶C的激活。
The effect of endothelin-1 (ET), a novel vasoactive peptide derived from endothelial cells, on osteoblastic MC3T3-E1 cells was studied. ET specifically binds to a single class of high-affinity receptors in MC3T3-E1 cells and induces phospholipase C activation with the production of two second messengers, inositol trisphosphate and 1,2-diacylglycerol, and a biphasic increase in intracellular free Ca2+ concentration ([Ca2+]i), which consists of an initial transient increase and an ensuing sustained plateau, as measured with a fluorescent indicator, fura-2. The second plateau phase but not the initial transient increase in [Ca2+]i induced by ET is abolished by removal of extracellular Ca2+ but not by either nicardipine, verapamil, or diltiazem. The ET-stimulated production of inositol trisphosphate is not abolished by removal of extracellular Ca2+, indicating that ET-stimulated phospholipase C activation is not a consequence of an increase in Ca2+ influx across the plasma membrane. ET causes stimulation of DNA synthesis and reduction of alkaline phosphatase activity in MC3T3-E1 cells. A protein kinase C activator phorbol 12,13-dibutyrate mimics these effects of ET. The results demonstrate that ET activates the inositol lipid signaling pathway and induces mobilization of Ca2+ from both extra- and intracellular pools and activation of protein kinase C in osteoblastic MC3T3-E1 cells.
内皮素作用：蛋白激酶 C 抑制剂的抑制和磷酸肌醇的参与。
DOI：
10.1016/s0006-291x(89)80193-7
发表时间：
1989
影响因子：
3.1
作者：
Sugiura,M;Inagami,T;Hare,GM;Johns,JA
通讯作者：
Johns,JA
影响因子：
3.1
作者：
Enright, E;Bland, AP;Kelly, AL
通讯作者：
Kelly, AL