Measles virus V protein blocks interferon (IFN)-alpha/beta but not IFN-gamma signaling by inhibiting STAT1 and STAT2 phosphorylation.
Measles virus V protein blocks interferon (IFN)-alpha/beta but not IFN-gamma signaling by inhibiting STAT1 and STAT2 phosphorylation.
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
K. Takeuchi;Shin-ichi Kadota;M. Takeda;N. Miyajima;K. Nagata
中科院分区：
文献类型：
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作者：
K. Takeuchi;Shin-ichi Kadota;M. Takeda;N. Miyajima;K. Nagata
麻疹病毒(MV)是副粘病毒科成员，编码C和V非结构蛋白。为了阐明MV C和V蛋白的功能，建立了组成性表达C和V蛋白的HeLa细胞系。我们发现V蛋白的表达抑制了干扰素-α/β信号转导，但不抑制干扰素-γ信号转导。在本实验条件下，C蛋白对干扰素信号转导无抑制作用。在表达V蛋白的HeLa细胞中，没有观察到选择性信号转导和转录激活子(STAT)蛋白的降解。相反，在干扰素-β刺激后，这些细胞中STAT1和STAT2的酪氨酸磷酸化被抑制。
Measles virus (MV), a member of the family Paramyxoviridae, encodes C and V non-structural proteins. To clarify the functions of MV C and V proteins, HeLa cell lines constitutively expressing C or V protein were established. We found that expression of V protein inhibited interferon (IFN)-alpha/beta signaling but not IFN-gamma signaling. C protein had no inhibitory effect on IFN signaling in our experimental condition. Degradation of selective signal transducers and activators of transcription (STAT) proteins was not observed in HeLa cells expressing V protein. In contrast, tyrosine phosphorylation of both STAT1 and STAT2 was inhibited in these cells after IFN-beta stimulation.