Differential expression of osteonectin/SPARC during human prostate cancer progression.
Differential expression of osteonectin/SPARC during human prostate cancer progression.
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发表时间：
2000-03
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通讯作者：
Regi Thomas;L. True;J. Bassuk;P. Lange;R. Vessella
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作者：
Regi Thomas;L. True;J. Bassuk;P. Lange;R. Vessella
前列腺癌（CaP）侵袭和转移的确切机制尚不清楚。骨连接蛋白 [ON（也称为 SPARC 或 BM-40）] 是一种抗粘附蛋白，已知参与细胞-基质相互作用、迁移和血管生成。在本报告中，我们研究了 ON 在人前列腺细胞系、原发性肿瘤和 CaP 转移灶中的表达。使用逆转录PCR和非放射性原位杂交(ISH)技术来测定ON基因表达。使用多克隆抗体LF37和/或单克隆抗体ON-mAb进行免疫组织化学。在正常组织的腺上皮细胞以及一些原代 CaP 中观察到低至中等水平的 ON mRNA 和蛋白质。然而，在大多数 CaP 转移灶（骨性和非骨性）中观察到高水平的 ON mRNA 和蛋白质。这与我们的发现很好地相关，即多种不同的 CaP 细胞系（包括来自转移瘤的四种 CaP 细胞系）显示出高水平的 ON 基因表达。此外，ISH 分析和细胞特异性逆转录 PCR 评估表明管腔细胞和基底细胞均表达 ON 基因。我们得出结论，ON 表达的差异模式表明它可能在 CaP 的进展中发挥重要作用。
The precise mechanism(s) involved in invasion and metastasis of prostate cancer (CaP) is poorly understood. Osteonectin [ON (also known as SPARC or BM-40)] is an antiadhesive protein known to be involved in cell-matrix interactions, migration, and angiogenesis. In this report, we studied the expression of ON in human prostate cell lines, primary tumors, and metastatic foci of CaP. Reverse transcription-PCR and nonradioactive in situ hybridization (ISH) techniques were used to determine ON gene expression. Immunohistochemistry was carried out using the polyclonal antibody LF37 and/or the monoclonal antibody ON-mAb. Low to moderate levels of ON mRNA and protein were observed in glandular epithelial cells of normal tissue as well as a few primary CaPs. However, high levels of ON mRNA and protein were observed in most of the CaP metastatic foci, both osseous and nonosseous. This correlated well with our findings that multiple different CaP cell lines including four CaP cell lines derived from metastases show high levels of ON gene expression. Furthermore, ISH analyses and cell-specific reverse transcription-PCR evaluation showed that both the luminal and basal cells express the ON gene. We conclude that the differential pattern of ON expression suggests that it may play an important role in the progression of CaP.