EFFECT OF VAGOTOMY ON EXPRESSION OF NEUROPEPTIDES AND HISTAMINE IN RAT OXYNTIC MUCOSA
EFFECT OF VAGOTOMY ON EXPRESSION OF NEUROPEPTIDES AND HISTAMINE IN RAT OXYNTIC MUCOSA
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DOI：
10.1007/bf02090208
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发表时间：
1994-02-01
影响因子：
3.1
通讯作者：
KIVILUOTO, T
中科院分区：
文献类型：
--
作者：
BACK, N;AHONEN, M;KIVILUOTO, T
本实验观察了迷走神经切断和幽门成形术对大鼠胃氧合酶粘膜内含蛙皮素/胃泌素释放肽(GRP)、降钙素基因相关肽(CGRP)、甘丙肽以及组胺、5-羟色胺(5-HT)和生长抑素阳性细胞的神经纤维密度的影响。切断迷走神经和幽门成形术后10天，胃氧合黏膜内含组胺的细胞密度增加了70%(P<；0.05)，这些细胞体积较大，突起较广。5-羟色胺免疫反应(IR)细胞密度和生长抑素IR细胞密度无明显变化。粘膜层CGRP-IR神经纤维密度明显减少，GRP-IR神经纤维密度略有下降，而肌层仅有少量CGRP-IR纤维减少，GRP-IR纤维未见减少。甘丙肽-IR神经纤维的密度不受影响。含氧粘膜高度降低约25%(P<；0.05)。因此，观察到对组胺-IR细胞的显著影响，支持了这些细胞受迷走神经调节的观点。研究还表明，胃底区粘膜层中的大部分CGRP-IR神经纤维和部分GRP-IR神经纤维来自迷走神经或受正常迷走神经活动的调节。
The effect of vagotomy and pyloroplasty on the density of nerve fibers containing bombesin/gastrin-releasing peptide (GRP), calcitonin gene-related peptide (CGRP), and galanin as well as histamine-, 5-hydroxytryptamine (5-HT)-, and somatostatin-containing cells in the oxyntic mucosa of the rat stomach was studied. Ten days after vagotomy and pyloroplasty the density of histamine-containing cells in the oxyntic mucosa was increased by 70% (P < 0.05), and these cells were larger and showed more extensive cell processes than in control animals. The density of 5-HT-immunoreactive (IR) cells and somatostatin-IR cells were not affected. A marked decrease in the density of CGRP-IR nerve fibers and a slighter decrease in the density of GRP-IR nerve fibers was observed in the mucosal layer while only a minor reduction of CGRP-IR fibers, and no reduction of GRP-IR fibers was seen in the muscular layer. The density of galanin-IR nerve fibers was not affected. The height of the oxyntic mucosa was reduced by about 25% (P < 0.05). Thus, a striking effect on the histamine-IR cells was seen, supporting the view that these cells are regulated by the vagus nerve. The study also indicates that a major portion of the CGRP-IR nerve fibers, and part of the GRP-IR nerve fibers, in the mucosal layer of the fundic region are of vagal origin or regulated by normal vagus nerve activity.