A point mutation of Adh1 gene is involved in the repression of coleoptile elongation under submergence in rice
A point mutation of Adh1 gene is involved in the repression of coleoptile elongation under submergence in rice
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DOI：
10.1270/jsbbs.56.69
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发表时间：
2006-03-01
期刊：
影响因子：
2.4
通讯作者：
Nakazono, M
中科院分区：
文献类型：
--
作者：
Saika, H;Matsumura, H;Nakazono, M
在高等植物中，需要酒精发酵来为糖酵解途径提供NAD（+），糖酵解途径负责在厌氧条件下合成ATP。Matsumura等人（1995）以前在水稻中分离出adh活性降低（ran）突变体，其中胚芽鞘的伸长在淹水时受到抑制。然而，rad基因尚未被表征。在本研究中，我们观察到，在胚芽鞘中，Adh 1的mRNA水平之间的rad突变体和野生型品种Kinmaze的可比性，而ADH 1蛋白的量是在rad突变体比野生型低得多。测序结果表明，突变株中Adh 1基因的G106被A所取代，导致推导的氨基酸序列中E36 K被替换。一个基因分型实验使用的F-2植物从一个交叉的rad突变体与籼稻品种Kasalath表明，点突变参与抑制胚芽鞘伸长。此外，由于ADH活性的降低似乎会导致ATP缺乏，胚芽鞘的伸长在淹没rad突变体中受到抑制。
in higher plants, alcoholic fermentation is required to supply NAD(+) to the glycolytic pathway, which is responsible for ATP synthesis under anaerobic conditions. Matsumura el al. (1995) previously isolated the reduced adh activity (ran) mutant in rice, in which elongation of the coleoptile is repressed tinder submergence. However, the rad gene had not been characterized. In the present Study, we observed that, in the coleoptile, the Adh1 mRNA levels were comparable between the rad mutant and the wild type cultivar Kinmaze, while the amount of ADH1 protein was much lower in the rad mutant than in the wild type. Sequencing showed that G106 of the Adh1 gene of Kinmaze was replaced with A in the rad mutant, resulting in an E36K substitution in the deduced amino acid sequence. A genotyping experiment using F-2 plants from a cross of the rad mutant with the indica cultivar Kasalath indicated that the point mutation was involved in the repression of coleoptile elongation. Furthermore, since the reduced ADH activity appeared to cause an ATP deficiency, elongation of the coleoptile was repressed in the Submerged rad mutant.