Expression of retinaldehyde dehydrogenase (RALDH)2 and RALDH3 but not RALDH1 in the developing anterior pituitary glands of rats
Expression of retinaldehyde dehydrogenase (RALDH)2 and RALDH3 but not RALDH1 in the developing anterior pituitary glands of rats
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DOI：
10.1007/s00441-006-0345-7
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发表时间：
2007-04-01
影响因子：
3.6
通讯作者：
Yashiro, Takashi
中科院分区：
文献类型：
--
作者：
Fujiwara, Ken;Maekawa, Fumihiko;Yashiro, Takashi
视黄酸（RA）在细胞生长和组织发育中发挥重要作用，也是垂体功能的调节因子。然而，RA是否在垂体中产生并发挥旁分泌和/或自分泌激素的作用目前尚不清楚。 RA 是由类维生素A 通过氧化过程合成的。催化视黄醛氧化为 RA 的脱氢酶是视黄醛脱氢酶 (RALDH) 家族的成员。在本研究中，我们检测了大鼠胚胎垂体中 RALDH1、RALDH2 和 RALDH3 mRNA 的表达。通过与地高辛标记的 cRNA 探针进行原位杂交，我们检测到 RALDH2 和 RALDH3 的 mRNA 表达，但未检测到 RALDH1。 RALDH2 和 RALDH3 的表达位于胚胎第 12.5 天 (E12.5) 的 Rathke 囊中，随后位于发育中的垂体前叶中。我们还使用实时定量聚合酶链反应来分析垂体发育过程中 RALDH2 和 RALDH3 mRNA 的表达水平。我们发现垂体 RALDH2 和 RALDH3 mRNA 水平在 E17.5 时较高，并在出生后显着下降。我们的研究首次表明，RALDH2 和 RALDH3 在大鼠胚胎垂体前叶中表达，但 RALDH1 不表达。
Retinoic acid (RA) plays an important role in cell growth and tissue development and is also a regulating factor of pituitary function. However, whether RA is generated in the pituitary gland and plays a role as a paracrine and/or autocrine hormone is generally unknown. RA is synthesized from retinoids through oxidation processes. Dehydrogenases catalyzing the oxidation of retinal to RA are members of the retinaldehyde dehydrogenase (RALDH) family. In this study, we examined the expression of RALDH1, RALDH2, and RALDH3 mRNA in the rat embryonic pituitary gland. By in situ hybridization with digoxigenin-labeled cRNA probes, we detected mRNA expression for RALDH2 and RALDH3, but not RALDH1. The expression of RALDH2 and RALDH3 was located in Rathke's pouch at embryonic day 12.5 (E12.5) and subsequently in the developing anterior pituitary gland. We also used quantitative real- time polymerase chain reaction to analyze RALDH2 and RALDH3 mRNA expression levels during the development of the pituitary gland. We found that pituitary RALDH2 and RALDH3 mRNA levels were high at E17.5 and decreased markedly after birth. Our study is the first to show that RALDH2 and RALDH3, but not RALDH1, are expressed in the embryonic anterior pituitary gland of the rat.