Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors
Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors
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DOI：
10.1073/pnas.0606631103
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发表时间：
2006-10-03
影响因子：
11.1
通讯作者：
Gleba, Yuri
中科院分区：
文献类型：
--
作者：
Giritch, Anatoli;Marillonnet, Sylvestre;Gleba, Yuri
植物病毒载体允许以高产量表达异源蛋白，但迄今为止，它们还不能有效地表达异源寡聚蛋白。我们在这里描述了一种快速和无限可扩展的方法，用于在植物中高水平表达IgG类的功能性全尺寸mAb。该过程依赖于两个病毒载体的同步共感染和共复制，每个载体表达单独的抗体链。这两种载体来源于两种不同的植物病毒，发现它们是非竞争性的。与来自相同病毒的载体不同，非竞争性载体在整个植物体的相同细胞中有效地共表达重链和轻链，导致每千克新鲜叶生物质的组装mAb的产量高达0.5 g。该技术允许在短短几天内生产克数量的单克隆抗体用于研究目的，并且在需要快速反应的情况下，例如大流行病或恐怖主义事件，可以在工业规模上使用相同的协议。
Plant viral vectors allow expression of heterologous proteins at high yields, but so far, they have been unable to express hetero-oligomeric proteins efficiently. We describe here a rapid and indefinitely scalable process for high-level expression of functional full-size mAbs of the IgG class in plants. The process relies on synchronous coinfection and coreplication of two viral vectors, each expressing a separate antibody chain. The two vectors are derived from two different plant viruses that were found to be noncompeting. Unlike vectors derived from the same virus, noncompeting vectors effectively coexpress the heavy and light chains in the same cell throughout the plant body, resulting in yields of up to 0.5 g of assembled mAbs per kg of fresh-leaf biomass. This technology allows production of gram quantities of mAbs for research purposes in just several days, and the same protocol can be used on an industrial scale in situations requiring rapid response, such as pandemic or terrorism events.